Anti-5-Hydroxymethylcytosine (5hmc) [AB3/63.3] mAb

The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine.

Contributors

Inventor Institute
Wolf Reik Babraham Institute
Cat. #: 152733
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Genetics;Stem cell biology
Application: IF ; IP ; DB
Target: 5-Hydroxymethylcytosine (5hmC)
Reactivity: All
Clone: AB3/63.3
Host: Rat
Class: Monoclonal
Product description: The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine. 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base. It is formed from the DNA base cytosine by adding a methyl group and then a hydroxy group. Every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; generally, the levels of 5-hydroxymethylcytosine increase with age. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethylcytosine may regulate gene expression or initiate DNA demethylation.
Conjugation: Unconjugated
Isotype: IgG2a kappa
Immunogen: 5-Hydroxymethylcytosine conjugated to BSA
Target background: The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine. 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base. It is formed from the DNA base cytosine by adding a methyl group and then a hydroxy group. Every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; generally, the levels of 5-hydroxymethylcytosine increase with age. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethylcytosine may regulate gene expression or initiate DNA demethylation.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -20° C
Shipping conditions: Dry ice
References:

Zhang et al. 2014. PLoS One. 9(1):e84925. PMID: 24427299.

Cao et al. 2014. Theriogenology. 81(3):496-508. PMID: 24315686.

Alterations of epigenetic signatures in hepatocyte nuclear factor 4a deficient mouse liver determined by improved ChIP-qPCR and (h)MeDIP-qPCR assays.

Dynamic reprogramming of 5-hydroxymethylcytosine during early porcine embryogenesis.

Ficz et al. 2011. Nature. 473(7347):398-402. PMID: 21460836.

Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation.

Wossidlo et al. 2011. Nat Commun. 2:241. PMID: 21407207.

5-Hydroxymethylcytosine in the mammalian zygote is linked with epigenetic reprogramming.

Images

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Anti-5-Hydroxymethylcytosine (5hmc) [AB3/63.3] has been used for hydroxymethylated DNA immunoprecipitation (hMeDIP) with enrichment of the target compared to negative controls. (Zhang et al. 2014. PLoS One. 9(1)

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