Cat. #161667
Anti-mouse Notch1
Cat. #: 161667
Sub-type: Primary antibody
Target: Notch1 protein
Class: Monoclonal
Application: Flow Cytometry, IP, WB, IHC-Fr, ICC
Reactivity: Human, Mouse.
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Laurie Milner
Institute: Fred Hutchinson Cancer Research Center
Primary Citation: Huppert et al.2000. Nature. 405[6789):966-70. PMID: 10879540
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-mouse Notch1
- Research fields: Cell biology
- Clone: Mn1a
- Tool sub type: Primary antibody
- Class: Monoclonal
- Reactivity: Human, Mouse.
- Host: Mouse
- Application: Flow Cytometry, IP, WB, IHC-Fr, ICC
- Description: Antibody mN1A recognizes Notch 1, one of the four major transmembrane receptors (Notch 1-4) of the Notch signaling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands.
- Isotype: IgG1
- Recommended controls: WB: HAP1 cell lysate Flow Cyt (Intra): SH-SY5Y cells, Jurkat cells.
Target Details
- Target: Notch1 protein
- Target background: Intracellular domain of Notch1 protein, mainly its activated form. The Notch genes encode single-pass transmembrane receptors that transduce the extracellular signals responsible for cell fate determination during several steps of metazoan development.
Applications
- Application: Flow Cytometry, IP, WB, IHC-Fr, ICC
- Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB)
Handling
- Storage conditions: Shipped at 4° C. Upon delivery aliquot and store at -20° C. Avoid freeze / thaw cycles.
References
- Huppert et al.2000. Nature. 405[6789):966-70.PMID: 10879540
