Anti-FCGR1 [10.1] mAb

Was created by Nancy Hogg at the London CRUK research institute: Lincoln’s Inn Fields, in order to mediate monocyte mediated antibody dependent lysis/death of cells/hybridomas expressing FCGR1 for IgG1. This means that high affinity FcR1 on monocytes can be used as a cytotoxic trigger to destroy targeted tumour cells expressing the antibody or heteroantibodies can be produced and used to target tumour cells which don’t express anti-FCGR1._x000D_ _x000D_ Research Application_x000D_ Many uses across a range of applications, especially immunotherapy and inflammation._x000D_ Involved in both innate and adaptive immune responses, cytokine production and phagocytosis._x000D_ _x000D_ Fc-gamma receptor 1 (CD64) is an…

Contributors

Inventor Institute
Nancy Hogg Cancer Research UK, Lincoln's Inn Fields Institute
Cat. #: 151026
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Cancer;Immunology
Application: FACS ; IHC ; IF ; IP ; Fn
Target: Fc-gamma receptor 1 (FCGR1, CD64)
Reactivity: Human
Clone: 10.1
Host: Mouse
Class: Monoclonal
Primary citation: Dougherty et al. 1987. Eur J Immunol. 17(10):1453-1459. PMID: 3500057. 
Alternate name: Fc Fragment Of IgG Receptor Ia; Fc Gamma Receptor Ia; Fc-Gamma RIA; IGFR1; FCRI; Fc Gamma Receptor; CD64 Antigen; FCGR1; CD64; FCG1
Product description: Anti-FCGR1 10.1 specifically recognises Fc-gamma receptor 1. This monoclonal antibody allows investigation of mononuclear phagocyte high affinity receptor, FcRI properties.
Conjugation: Unconjugated
Isotype: IgG1
Molecular weight: 71 kDa
Immunogen: Rheumatoid synovial fluid cells and fibronectin purified human monocytes.
Immunogen Uniprot ID: P12314
Myeloma used: Sp2/0-Ag14
Target background: Was created by Nancy Hogg at the London CRUK research institute: Lincoln's Inn Fields, in order to mediate monocyte mediated antibody dependent lysis/death of cells/hybridomas expressing FCGR1 for IgG1. This means that high affinity FcR1 on monocytes can be used as a cytotoxic trigger to destroy targeted tumour cells expressing the antibody or heteroantibodies can be produced and used to target tumour cells which don't express anti-FCGR1. Research Application Many uses across a range of applications, especially immunotherapy and inflammation. Involved in both innate and adaptive immune responses, cytokine production and phagocytosis. Fc-gamma receptor 1 (CD64) is an IgG receptor expressed by monocytes and myeloid cell lines. It is a marker of inflammation. It is involved in cancer and many chronic diseases (arthritis, multiple sclerosis, diabetic ulcers), but also in antibody-dependent cell-mediated cytotoxicity (ADCC), sepsis, automimmune disorders and periodic fever, aphthous stomatitis, pharyngitis, and cervical adenitis (PFAPA) syndrome. The 10.1 antibody blocks the Ig binding to FcR1 receptor.
Format: Liquid
Concentration: 0.97 mg/ml
Storage buffer: PBS containing 0.02% Sodium Azide.
Storage conditions: Store undiluted 2° C to 8° C, do not freeze and protect from prolonged light exposure
Shipping conditions: Dry ice
References:

Onuora et al. 2019. Nat Rev Rheumatol. 15(8):450. PMID: 31278378.

Rogers et al. 2017. Cytometry B Clin Cytom. 92(4):258-265. PMID: 26566003.

Sips et al. 2016. Mucosal Immunology. 9(6):1584-1595. PMID: 26883728.

Tan et al. 2016. Eur J Immunol. 46(4):919-927. PMID: 26763072.

Hanihara et al. 2016. J Neurosurg. 124(6):1-8. PMID: 26636389.

Tutt et al. 2015. J Immunol. 195(11):5503-5516. PMID: 26512139.

Schwab et al. 2015. Cell Rep. 13(3):610-620. PMID: 26456831.

Sousa et al. 2015. Breast Cancer Res. 17(1):101. PMID: 26243145.

Conrad et al. 2015. Arthritis Res Ther. 17(1):1-12. PMID: 26178906.

Matt et al. 2015. Scand J Rheumatol. 44(6):464-473. PMID: 26084203.

Pilling et al. 2015. PLoS One. 10(3):e0119709. PMID: 25774777.

Sutherland et al. 2015. Curr Protoc Cytom. 72(1):6.37.1-6.37.29. PMID: 25827482.

Mancardi et al. 2013. Blood. 121(9):1563-73. PMID: 23293080.

Schleinitz et al. 2008. Arthritis Rheum. 58(10):3216-23. PMID: 18821690.

Jayaram et al. 1989. Clin Exp Immunol. 75(3):414-20. PMID: 2495203.

Dougherty et al. 1987. Eur J Immunol. 17(10):1453-1459. PMID: 3500057.

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