3LL NRAS KO #63 cell line

Murine Lewis lung adenocarcinoma engineered cells represent a model for study of the effect of KRAS-G12C inhibitors on oncogenic cells. The cell line was established from 3LL cells by NRAS…

Contributors

Inventor Institute
Julian Downward The Francis Crick Institute
Cat. #: 157771
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Organism: Mouse
Tissue: Lung
Gender: Male
Model: Knock-Out
Growth properties: Semi-adherent
Primary citation: Molina-Arcas et al. 2019. Sci Transl Med. 11(510). PMID: 31534020
Alternate name: 3LL ΔNRAS
Product description: Murine Lewis lung adenocarcinoma engineered cells represent a model for study of the effect of KRAS-G12C inhibitors on oncogenic cells. The cell line was established from 3LL cells by NRAS knocking out using CRISPR-Cas9 to promote a stronger dependency on oncogenic G12C KRAS. It was used in development of combination therapies to maximize the impact of G12C KRAS inhibitors in lung cancer
Gender: Male
CRISPR: Yes
Conditional: No
Production details: NRAS CRISPR/Cas knockout: To knock out NRAS, 3LL cells were transiently transfected with the pSpCas9(BB)-2A-GFP vector (PX458, Addgene) expressing a GFP expression cassette and with the gRNA 5’-gRNA-‘3 ACTGGACACAGCTGGACATG, PAM: ATG. GFP-positive cells were sorted using a MoFlo XDP sorter and single cell cloned manually. Clones were amplified and NRAS protein was quantified by Western blot
Additional notes: CRISPR edited 3LL cells.Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here.This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide these modified CRISPR-Cas9 cell lines to companies under a label…
Parental cell line: 3LL aka LL/2 (LLC1) likely origin – the authors have established (by DNA sequencing) that these cells are homozygous for the KRAS-G12C & NRAS-Q61H mutations which is not the case for the parent 3LL ATCC line
Model description: NRAS KO using CRISPR/Cas9 to promote a stronger dependency on oncogenic G12C KRAS
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: RPMI + 10% FCS + 1% Penicillin/Streptomycin
Mycoplasma free: Yes
Biosafety level: 1
References:

Molina-Arcas et al. 2019. Sci Transl Med. 11(510). PMID: 31534020

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