YIp211-P30-His-pol30(K127R)-3′ Plasmid

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; URA3 marker. Proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase’s processibility during elongation of the leading strand. Involved in DNA repair.

Contributors

Institute
Cancer Research UK London Research Institute: Clare Hall Laboratories
Cat. #: 153616
Tool sub type: YIplac211
Research Fields: Cell biology;Genetics;Tags and cell markers
Target: POL30 (<i>Saccharomyces cerevisiae</i>)
Backbone size without insert: 3797
Bacterial resistance: Ampicillin
Selectable markers: URA3
Alternate name: PCNA, Proliferating cell nuclear antigen
Product description: Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; URA3 marker. Proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair.
Additional notes: Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; URA3 marker. Proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair.
References:

Detection of PCNA modifications in Saccharomyces cerevisiae. Davies AA, Ulrich HD Methods Mol Biol. 2012

920:543-67. doi: 10.1007/978-1-61779-998-3_36.

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