Cat. #151809
2fTGH-U4A Cell Line
Cat. #: 151809
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Human
Disease: Cancer
Model: Mutant
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: George Stark
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: 2fTGH-U4A Cell Line
- Cancer: Sarcoma
- Cancers detailed: Fibrosarcoma
- Research fields: Cancer;Cell signaling and signal transduction;Immunology
- Tool sub type: Continuous
- Parental cell: HT 1080
- Organism: Human
- Disease: Cancer
- Model: Mutant
- Conditional: Yes
- Description: The 2fTGH-U4A Cell Line is part of a panel of IFNy pathway mutant fibrosarcoma cell lines isolated by chemical mutagenesis of IFNy insensitive reporter cells derived from HT1080 cells. Knockout genes have been identified and span multiple members of the IFMy pathway. These cell lines are be useful for the in vitro study and comparison of disrupted interferon signalling at multiple points across the IFN pathway. The following cell lines are part of the group of IFN signalling mutants: U4C, U2A, U3A, 2FTGH, U6A, U5A. Each containing a different mutation in the IFN signalling pathway.
- Production details: Human; HT1080 human sarcoma cell lines transfected with a vector encoding a selectable marker regulated by interferon to create the 2fTGH cell line, enabling selection of mutations in genes encoding components of the interferon signalling pathway. Chemical mutagenesis of the 2fTGH cell line enabled isolation of 10 IFNg signalling mutants.
- Recommended controls: The wild type 2FTGH human fibrosarcoma as a positive control together with the U5a and U3a IFNB resistant cell lines.
- Cellosaurus id: CVCL_9470
Target Details
- Target: IFN signalling mutant U4A
Handling
- Format: Frozen
- Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.
Related Tools
- Related tools: 2fTGH-U2A Cell Line ; 2fTGH-U3A Cell Line ; 2fTGH Cell Line ; 2fTGH-U6A Cell Line ; 2fTGH-U4C Cell Line ; 2fTGH-U5A Cell Line
References
- Haan et al. 2008. J Immunol. 180(2):998-1007. PMID: 18178840.
- Dual role of the Jak1 FERM and kinase domains in cytokine receptor binding and in stimulation-dependent Jak activation.
- Sun et al. 2004. J Interferon Cytokine Res. 24(6):350-61. PMID: 15212709.
- Ectopic expression of toll-like receptor-3 (TLR-3) overcomes the double-stranded RNA (dsRNA) signaling defects of P2.1 cells.
- Guo et al. 2000. Virology. 267(2):209-19. PMID: 10662616.
- Induction of the human protein P56 by interfero...
