2fTGH-U5A Cell Line

The 2fTGH-U5A Cell Line is part of a panel of IFNy pathway mutant fibrosarcoma cell lines isolated by chemical mutagenesis of IFNy insensitive reporter cells derived from HT1080 cells. Knockout…

Contributors

Inventor Institute
Ian Kerr Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 151808
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Organism: Human
Gender: Male
Model: Mutant
Product description: The 2fTGH-U5A Cell Line is part of a panel of IFNy pathway mutant fibrosarcoma cell lines isolated by chemical mutagenesis of IFNy insensitive reporter cells derived from HT1080 cells. Knockout genes have been identified and span multiple members of the IFMy pathway. These cell lines are be useful for the in vitro study and comparison of disrupted interferon signalling at multiple points across the IFN pathway. The following cell lines are part of the group of IFN signalling mutants: U4C, U2A, U3A, 2FTGH, U6A, U5A. Each containing a different mutation in the IFN signalling pathway.
Gender: Male
CRISPR: Yes
Conditional: Yes
Production details: Human; HT1080 human sarcoma cell lines transfected with a vector encoding a selectable marker regulated by interferon to create the 2fTGH cell line, enabling selection of mutations in genes encoding components of the interferon signalling pathway. Chemical mutagenesis of the 2fTGH cell line enabled isolation of 10 IFNg signalling mutants.
Cellosaurus ID: CVCL_D315
Parental cell line: HT 1080
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Parental 2fTGH and mutant cell lines can be grown in DMEM with 10% FCS.
Recommended controls: The wild type 2fTGH human fibrosarcoma as a positive control together with the U5a and U3a IFNB resistant cell lines.
Mycoplasma free: Yes
References:

Haan et al. 2008. J Immunol. 180(2):998-1007. PMID: 18178840.

Dual role of the Jak1 FERM and kinase domains in cytokine receptor binding and in stimulation-dependent Jak activation.

Sun et al. 2004. J Interferon Cytokine Res. 24(6):350-61. PMID: 15212709.

Ectopic expression of toll-like receptor-3 (TLR-3) overcomes the double-stranded RNA (dsRNA) signaling defects of P2.1 cells.

Guo et al. 2000. Virology. 267(2):209-19. PMID: 10662616.

Induction of the human protein P56 by interfero…

Images

View Gallery
2fTGH-U5A Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

Related Tools for Cell lines

Cat. # Tool Name
151488 B6.1.1 Mouse ES Cell Line Key Info View Tool
151626 A375P beta6-puro Cell Line Key Info View Tool
151665 MEF PKCe KO Cell Line Key Info View Tool
151014 MCF7 AREc32 Cell Line Key Info View Tool
151655 FLYA-13 Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.