pBABE-hygro Vector

High-titer retroviral empty backbone pBABE vector, ready for cloning. pBABE-hygro enables stable gene delivery and expression in mammalian cells with MO-MuLV LTR-driven expression for reliable, long-term transgene production. Features Hygromycin B selection useful for sequential selection studies. pBABE-hygro is a part of the established pBABE vector system, developed by Morgenstern and Land 1990. Key features […]

Contributors

Inventor Institute
Hartmut Land, Jay Morgenstern Cancer Research UK London Research Institute: Lincoln's Inn Fields
Cat. #: 162284
Tool sub type: 10XUAS IVS p10
Unit size: Provided as 50-100ng cloning grade DNA on Whatman filter paper
Research Fields: Genetics;Genetics
Total vector size: 5558
Backbone size without insert: 5558
Bacterial resistance: Ampicillin
Selectable markers: Hygromycin
Primary citation: Morgenstern et al. 1990. Nucleic Acids Res. 18(12):3587-96. PMID: 2194165.
Product description: High-titer retroviral empty backbone pBABE vector, ready for cloning. pBABE-hygro enables stable gene delivery and expression in mammalian cells with MO-MuLV LTR-driven expression for reliable, long-term transgene production. Features Hygromycin B selection useful for sequential selection studies. pBABE-hygro is a part of the established pBABE vector system, developed by Morgenstern and Land 1990. Key features include: high viral titers for efficient transduction, stable long-term gene expression via LTR promoter and helper-free design which minimises contamination risk.
Additional notes: The pBABE vectors prove useful in experiments where highest frequencies of gene transfer, or concomitant expression of several different genes within a single cell are required with minimal risk of helper virus contamination.
Shipping conditions: Provided as 50-100ng cloning grade DNA on Whatman filter paper
Plasmid amplification details: DH5alpha 37° C
Plasmid copy number: High copy

Images

View Gallery
Vector map

Citations And Reviews

Related Tools for Vectors

Cat. # Tool Name
152050 pJEK21 Gamma-Synuclein Vector Key Info View Tool
152051 pJEK6 Alpha-Synuclein E46K Vector Key Info View Tool
153287 pCMV-iNOS Vector Key Info View Tool
153458 pRSF-1b-UbcH5B Vector Key Info View Tool
153547 pHyg-AID(31-114)-9myc Plasmid Key Info View Tool

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.