A549 CisR cell line

Cisplatin resistant (CisR) Non-Small Cell Lung Cancer Cell lines

Contributors

Inventor Institute
Martin P. Barr Trinity College Dublin
Cat. #: 162205
Tool sub type: 10XUAS IVS p10
Organism: Human
Tissue: Lung
Gender: Male
Model: Therapy Resistance / Drug (Cisplatin) / Extrinsic
Morphology: Epithelial
Growth properties: Adherent
Primary citation: Barr MP, et al. 2013. PLoS One. 8(1):e54193. PMID: 23349823
Alternate name: A549 Chemo-resistant cell line
Product description: The developmen of acquired cisplatin resistance is a significant clinical challenge in the treatment of NSCLC patients. An isogenic panel of cisplatin resistant (CisR) A549 cell lines were generated from their wild-type/parental (PT) counterparts and extensively characterised. These provide a valuable in vitro tool for investigating molecular pathways and mechanisms of resistance that may be associated with the cisplatin resistant phenotype in lung cancer patients.
Gender: Male
CRISPR: Yes
Conditional: Yes
Parental cell line: A549
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: Hams-F12 media supplemented with 10% FBS, 1% Penicillin/Streptomycin, 4mM L-Glutamine (final concentration)
Subculture routine: Subculture cells using trypsin-EDTA. Neutralise trypsin in supplemented cell culture media and pellet cells by centrifugation at 1300 rpm for 3 min. Discard supernatant to waste and resuspend the cell pellet in 1 mL of supplmented cell culture media. Transfer cell content to a T75 cm2 vented culture flask and incubate until further use.
Atmosphere: 5% CO2
Mycoplasma free: Yes

Citations And Reviews

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