#161590

IPTG-inducible pGEX-6P1 expression vector for GST and human CB1 receptor (AA 414 to 472) fusion protein

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Cat. #161590

IPTG-inducible pGEX-6P1 expression vector for GST and human CB1 receptor (AA 414 to 472) fusion protein

Cat. #: 161590

Sub-type: IPTG-inducible pGEX-6P1 expression vector

Bacterial Resistance: Ampicillin

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Gontzal García del Caño

Institute: University of the Basque Country

Primary Citation: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.

Tool Details
Handling
Application Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: IPTG-inducible pGEX-6P1 expression vector for GST and human CB1 receptor (AA 414 to 472) fusion protein
  • Research fields: Biochemistry;Cell biology;Molecular Biology;Neurobiology
  • Tool sub type: IPTG-inducible pGEX-6P1 expression vector
  • Sequence: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
  • Insert: GST-CB1414–472
  • Tag: glutathione S-transferase (GST)
  • Total vector size: 5138 bp
  • Backbone size without insert: 4984 bp
  • Insert size: 183 bp
  • Insert species: Human
  • Promoter: Isopropyl ?-D-thiogalactoside (IPTG)-inducible tac promoter. The vector is designed with an internal lacIq gene whose protein product binds to the operator region of the tac promoter. Thus, expression is repressed unless IPTG is added to the culture, thus maintaining tight control over the expression of the insert.
  • Bacterial resistance: Ampicillin
  • Description: Purified plasmid DNA containing the IPTG inducible coding sequence for the polypeptide spanning amino acids 414 to 472 of the cytosolic tail of the human CB1 receptor fused to the C-terminus of the glutathione S-transferase tag (GST-CB1414–472)
  • Application: GST fusion protein production
  • Cloning information: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
  • Additional notes: Contact the inventor for further information.

Handling

  • Storage conditions: Refrigerated at 4 oC (or frozen at -20 oC for long periods)
  • Storage buffer: Double distilled water
  • Shipping conditions: At room temperature in water solution

Application Details

  • Application: GST fusion protein production
  • Application notes: GST fusion protein production

References

  • Saumell-Esnaola et al. 2022 Microb Cell Fact.21(1):192. PMID: 36109736
  • Gómez-Caballero et al. 2021. Mikrochim Acta. 188(11):368. PMID: 34618242.