Instituto de Medicina Molecular JoĂÂÄšÂo Lobo Antunes
SKU:
160728
Product description:
Venus-A2AR construct was generated with the In-fusion HD Cloning Kit (Clontech Takara, USA). Venus and A2AR fragments were produced by using, respectively, the pair of primers 5′-GTTTAAACTTAAGCTTATGGTGAGCAAGGGCGAG-3′ and 5′-GCTGCCCATGGTGGCCTTGTACAGCTCGTCCATG-3′, and the pair of primers 5′-GCCACCATGGGCAGCAGC-3′ and 5′-AAACGGGCCCTCTAGATCAGCTGGGGGCGAACTC-3′. PCR fragments were cloned into the vector pcDNA3.1(ÄËĂÂĂÂ+ÄËĂÂĂÂ) linearized with HindIII and XbaI, and the resulting construct was verified by DNA sequencing (GATC Biotech, Germany).
Alternate name:
A2AR
Additional notes:
A construct encoding a Venus-A2AR fusion protein to explore the synaptic function of A2AR
Cat. #:
160728
Tool sub type:
pcDNA 3.1
Research Fields:
Cell biology; Neurobiology
Target:
Adenosine A2A receptor
Bacterial resistance:
Ampicillin
Selectable markers:
Neomycin
Application notes:
Venus-A2AR construct was generated with the In-fusion HD Cloning Kit (Clontech Takara, USA). Venus and A2AR fragments were produced by using, respectively, the pair of primers 5′-GTTTAAACTTAAGCTTATGGTGAGCAAGGGCGAG-3′ and 5′-GCTGCCCATGGTGGCCTTGTACAGCTCGTCCATG-3′, and the pair of primers 5′-GCCACCATGGGCAGCAGC-3′ and 5′-AAACGGGCCCTCTAGATCAGCTGGGGGCGAACTC-3′. PCR fragments were cloned into the vector pcDNA3.1( + ) linearized with HindIII and XbaI, and the resulting construct was verified by DNA sequencing (GATC Biotech, Germany).
References:
Temido-Ferreira et al. 2020. Mol Psychiatry. 25(8):1876-1900. PMID: 29950682.
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