Venus-A2AR vector

Venus-A2AR construct was generated with the In-fusion HD Cloning Kit (Clontech Takara, USA). Venus and A2AR fragments were produced by using, respectively, the pair of primers 5′-GTTTAAACTTAAGCTTATGGTGAGCAAGGGCGAG-3′ and 5′-GCTGCCCATGGTGGCCTTGTACAGCTCGTCCATG-3′, and the pair of primers 5′-GCCACCATGGGCAGCAGC-3′ and 5′-AAACGGGCCCTCTAGATCAGCTGGGGGCGAACTC-3′. PCR fragments were cloned into the vector pcDNA3.1( + ) linearized with HindIII and XbaI, and the resulting construct was verified by DNA sequencing (GATC Biotech, Germany).