| Inventor | Institute |
|---|---|
| Prof Emanuele Buratti | International Centre For Genetic Engineering And Biotechnology (ICGEB) |
| SKU: | 153793 |
|---|---|
| Product description: | Concentration 1.35mg/ml |
| Additional notes: | This vector is a typical hybrid minigene that contains a ‘‘simplified’’ version of the gene that will be evaluated for pre-mRNA splicing. In particular, it contains at the 5′ end an alfa-globin gene promoter and SV40 enhancer sequences to allow polymerase II transcription in the transfected cell lines. This is followed by a series of exonic and intronic sequences that may derive from a reporter gene or from the gene context itself. In this case, the reporter gene is composed of a-globin and fibronectin exons, while at the 3′ end a functionally competent polyadenylation site, derived from the alfa-globin gene, is present. The genomic DNA region of interest that contains a putative splicing mutation is introduced into the minigene in a unique restriction site (NdeI). |
| Cat. #: | 153793 |
|---|---|
| Tool sub type: | pBluescript KS |
| Research Fields: | Genetics |
| Backbone size without insert: | 2958 |
| Bacterial resistance: | Ampicillin |
| Application notes: | Concentration 1.35mg/ml |
|---|
| References: |
Pagani et al. 2000. J Biol Chem. 275(28):21041-7. PMID: 10766763. Splicing factors induce cystic fibrosis transmembrane regulator exon 9 skipping through a nonevolutionary conserved intronic element. |
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