pTB_CFTR_Ex9 155T vector

Contributors

Inventor Institute
Prof Emanuele Buratti International Centre For Genetic Engineering And Biotechnology (ICGEB)
SKU: 153794
Product description: This minigene construct consists of a minimal alpha globin promoter and SV40 enhancer which drive the transcription of the minigene. Downstream, an alpha-globin-fibronectin EDB minigene is present with a unique NdeI site where a fragment that contains exon 9 (183bp) along with part of the flanking introns was inserted. The exon 9 sequence also carries a C155T mutation that destroys a splicing enhancer within its sequence. This means that when transfected into cells the exon 9 is included approximately in 50% of the transcripts. In this manner, it is possible to see changes both with regards to upregulation of exon inclusion or its downregulation. Concentration 2ug/ml
Alternate name: Cystic Fibrosis Transmembrane Conductance Regulator, Channel Conductance-Controlling ATPase
Additional notes: This minigene construct consists of a minimal alpha globin promoter and SV40 enhancer which drive the transcription of the minigene. Downstream, an alpha-globin-fibronectin EDB minigene is present with a unique NdeI site where a fragment that contains exon 9 (183bp) along with part of the flanking introns was inserted. The exon 9 sequence also carries a C155T mutation that destroys a splicing enhancer within its sequence. This means that when transfected into cells the exon 9 is included approximately in 50% of the transcripts. In this manner, it is possible to see changes both with regards to upregulation of exon inclusion or its downregulation.
Cat. #: 153794
Tool sub type: pBluescript KS
Research Fields: Genetics
Target: CFTR Exon 9 minigene
Backbone size without insert: 2958
Bacterial resistance: Ampicillin
Application notes: Concentration 2ug/ml
References:

D'Ambrogio et al. 2009. Nucleic Acids Res. 37(12):4116-26. PMID: 19429692.

Functional mapping of the interaction between TDP-43 and hnRNP A2 in vivo.

Pagani et al. 2003. J Biol Chem. 278(29):26580-8. PMID: 12732620.

Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9.

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A schematic diagram of the CFTR C155T minigene

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