| Inventor | Institute |
|---|---|
| Dr. Patrick Loll | Drexel University |
| SKU: | 156511 |
|---|---|
| Product description: | Affinity-tagged small ubiquitin related modifier (SUMO) fusion proteins generated by the cloning vectors: pETHSUL, pETS2SUL, pASHSUL, or pASS2SUL are treated with recombinant SUMO protease, generated from the pdtUD1 vector, to cleave SUMO and yield the mature target protein. Instructions for vector expression, purification, and use are outlined in: Weeks, S. D., Drinker, M., & Loll, P. J. (2007). Ligation independent cloning vectors for expression of SUMO fusions. Protein expression and purification, 53(1), 40ÄËĂÂĂÂ50. doi:10.1016/j.pep.2006.12.006 Protein Purification; Protein Biochemistry |
| Additional notes: | The pdtUD1 vector allows the production of recombinant UD1 domain of the S. cerevisiae Ulp1 protein (small ubiquitin related modifier (SUMO) protease). The UD1 domain retains the full SUMO-specific proteolytic activity. The recombinant protease has an amino terminal Strep-II tag and a carboxy terminal His6 tag to allow for easy purification of the recombinant protease. The dual tags also allow the protease to be removed after cleavage of the SUMO fusion protein generated by the cloning vectors: pETHSUL, pETS2SUL, pASHSUL, or pASS2SUL, by using subtractive affinity chromatography. |
| Cat. #: | 156511 |
|---|---|
| Tool sub type: | pETSU vector containing an untagged yeast SUMO sequence |
| Research Fields: | Other |
| Target: | A dual tagged clone of the catalytic domain (dtUD1) of the S. cerevisiae SUMO hydrolase. The recombinant protease has an amino terminal Strep-II tag and a carboxy terminal His6 tag. |
| Bacterial resistance: | Ampicillin |
| Application notes: | Affinity-tagged small ubiquitin related modifier (SUMO) fusion proteins generated by the cloning vectors: pETHSUL, pETS2SUL, pASHSUL, or pASS2SUL are treated with recombinant SUMO protease, generated from the pdtUD1 vector, to cleave SUMO and yield the mature target protein. Instructions for vector expression, purification, and use are outlined in: Weeks, S. D., Drinker, M., & Loll, P. J. (2007). Ligation independent cloning vectors for expression of SUMO fusions. Protein expression and purification, 53(1), 40–50. doi:10.1016/j.pep.2006.12.006 Protein Purification; Protein Biochemistry |
|---|
| References: |
Weeks et al. 2007. Protein Expr Purif. 53(1):40-50. PMID: 17251035. |
|---|
Please note we may take up to three days to respond to your enquiry.
CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.