Dicer; miRNA; miRNA biogenesis; reporter assay; high-throughput screening
| Inventor | Institute |
|---|---|
| Andrii Domanskyi, Piotr Chmielarz, Ilmari Parkkinen | University of Helsinki |
| SKU: | 161705 |
|---|---|
| Product description: | DARA plasmid was created for the Dicer activation reporter assay, which is used to screen compounds for miRNA biogenesis activation. Dicer has been implicated in certain cancers, ALS and Parkinsons Disease. The DARA plasmid was designed to express three main elements: 1) a primary fluorescent reporter protein, a tandem dimer Tomato (tdTomato) red fluorescent protein, which additionally has a nuclear localization sequence (NLS), 3’ untranslated sequence (3’UTR) containing miRNA miR-19b bin… |
| Alternate name: | pTO-miR19b-tdTom-19b_bind-WPRE-EGFP Dicer-activating reporter assay (DARA) plasmid |
| Cat. #: | 161705 |
|---|---|
| Research Fields: | Cell biology |
| Application: | High throughput screening of chemical compound libraries |
| Total vector size: | 9990 bp |
| Backbone size without insert: | 4329 bp |
| Bacterial resistance: | Ampicillin |
| Selectable markers: | Hygromycin |
| Primary citation: | N/A |
| Application: | High throughput screening of chemical compound libraries |
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| Shipping conditions: | +4° C or dry ice |
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| Plasmid amplification details: | Stbl3 E.coli |
| Plasmid copy number: | High copy number |
| References: |
N/A |
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