Instituto de Medicina Molecular JoĂÂÄšÂo Lobo Antunes
SKU:
158344
Product description:
The two reporter sequences (for GTS-mCherry and H2B-GFP) are linked by the 2A self-cleaving peptide to allow expression of stoichiometric amounts of both fluorescent reporters.
The 2A peptide-linked multicistronic vectors can be used to express multiple proteins from a single open reading frame (ORF).
The small 2A peptide sequence, when cloned between genes, allows for efficient, stoichiometric production of discrete protein products within a single vector through a novel “cleavage” event within the 2A peptide sequence. 2A peptides are between 18-22 amino acids in length and have divergent amino-terminal sequences, (minimising the chance for homologous recombination. For more information see reference PMID: 22301656
Cat. #:
158344
Research Fields:
Cell biology
Application notes:
The two reporter sequences (for GTS-mCherry and H2B-GFP) are linked by the 2A self-cleaving peptide to allow expression of stoichiometric amounts of both fluorescent reporters.
The 2A peptide-linked multicistronic vectors can be used to express multiple proteins from a single open reading frame (ORF).
The small 2A peptide sequence, when cloned between genes, allows for efficient, stoichiometric production of discrete protein products within a single vector through a novel “cleavage” event within the 2A peptide sequence. 2A peptides are between 18-22 amino acids in length and have divergent amino-terminal sequences, (minimising the chance for homologous recombination. For more information see reference PMID: 22301656
References:
Barbacena et al. 2019. Genesis. 57(6):e23299. PMID: 30990965.
Szymczak-Workman et al. 2012. Cold Spring Harb Protoc. 2012(2):199-204. PMID: 22301656.
Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.
Please note we may take up to three days to respond to your enquiry.
CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.
