pUC57-2A vector

The two reporter sequences (for GTS-mCherry and H2B-GFP) are linked by the 2A self-cleaving peptide to allow expression of stoichiometric amounts of both fluorescent reporters. The 2A peptide-linked multicistronic vectors can be used to express multiple proteins from a single open reading frame (ORF). The small 2A peptide sequence, when cloned between genes, allows for […]

Contributors

Inventor Institute
Dr Claudio Franco Instituto de Medicina Molecular JoĂŁo Lobo Antunes
Cat. #: 158344
Research Fields: Cell biology
Product description: The two reporter sequences (for GTS-mCherry and H2B-GFP) are linked by the 2A self-cleaving peptide to allow expression of stoichiometric amounts of both fluorescent reporters. The 2A peptide-linked multicistronic vectors can be used to express multiple proteins from a single open reading frame (ORF). The small 2A peptide sequence, when cloned between genes, allows for efficient, stoichiometric production of discrete protein products within a single vector through a novel "cleavage" event within the 2A peptide sequence. 2A peptides are between 18-22 amino acids in length and have divergent amino-terminal sequences, (minimising the chance for homologous recombination. For more information see reference PMID: 22301656
Application notes: The two reporter sequences (for GTS-mCherry and H2B-GFP) are linked by the 2A self-cleaving peptide to allow expression of stoichiometric amounts of both fluorescent reporters. The 2A peptide-linked multicistronic vectors can be used to express multiple proteins from a single open reading frame (ORF). The small 2A peptide sequence, when cloned between genes, allows for efficient, stoichiometric production of discrete protein products within a single vector through a novel "cleavage" event within the 2A peptide sequence. 2A peptides are between 18-22 amino acids in length and have divergent amino-terminal sequences, (minimising the chance for homologous recombination. For more information see reference PMID: 22301656
References:

Barbacena et al. 2019. Genesis. 57(6):e23299. PMID: 30990965.

Szymczak-Workman et al. 2012. Cold Spring Harb Protoc. 2012(2):199-204. PMID: 22301656.

Images

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The pUC57-2A plasmid coding for GTS-mCherry and H2B-GFP sequences.

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