pROSA-GUIDE

Validated gRNA targeting ROSA26 locus in mouse cells. Use in combination with pROSA-puro-GFP CRISPR or pROSA-puro-iRFP720 CRISPR to integrate any gene of interest at ROSA26 locus in mouse cells. gRNA in plentiCRISPRv1 backbone. gRNA sequence based on Quadros et al 2015 PMID: 25834785. Species: Mus musculus (house mouse) Cloning method: Restriction Enzyme 5’ cloning site: BsmbI 3’ cloning site: BsmbI 5’ sequencing primer: U6 Forward

Contributors

Inventor Institute
Dr Andrew Stephens ; Amy Wilson Hudson Institute Of Medical Research
SKU: 153872
Product description: Validated gRNA targeting ROSA26 locus in mouse cells. Use in combination with pROSA-puro-GFP CRISPR or pROSA-puro-iRFP720 CRISPR to integrate any gene of interest at ROSA26 locus in mouse cells. gRNA in plentiCRISPRv1 backbone. gRNA sequence based on Quadros et al 2015 PMID: 25834785. Species: Mus musculus (house mouse) Cloning method: Restriction Enzyme 5’ cloning site: BsmbI 3’ cloning site: BsmbI 5’ sequencing primer: U6 Forward
Cat. #: 153872
Tool sub type: Mammalian Expression, Lentiviral, CRISPR
Research Fields: Cell biology; Genetics
Target: Gt(ROSA)26Sor; ROSA26
Backbone size without insert: 11530
Bacterial resistance: Ampicillin
Selectable markers: Puromycin
Application notes: Validated gRNA targeting ROSA26 locus in mouse cells. Use in combination with pROSA-puro-GFP CRISPR or pROSA-puro-iRFP720 CRISPR to integrate any gene of interest at ROSA26 locus in mouse cells. gRNA in plentiCRISPRv1 backbone. gRNA sequence based on Quadros et al 2015 PMID: 25834785. Species: Mus musculus (house mouse) Cloning method: Restriction Enzyme 5’ cloning site: BsmbI 3’ cloning site: BsmbI 5’ sequencing primer: U6 Forward

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