Methionine Deficient Green Florescent Protein (mGFP) vector

Methionine Deficient Green Florescent Protein (mGFP) is a mutated form of GFPuv (from Aequorea victoria) a GFP variant optimised for maximal fluorescence when excited by UV light. GFPuv can be used as a fusion partner to assist in the expression and isolation of peptides and to monitor biological processes. When GFPuv is fused to other proteins/peptides cyanogen bromide (CNBr) has been used to cleave the fused proteins at methionine residues. However, purification of the protein becomes more complex as the number of methionine residues in the reporter protein increases. GFPuv has four methionine residues which will lead to five fragments…

Contributors

Inventor Institute
Dr. Bob Beitle University of Arkansas, Fayetteville
SKU: 156390
Product description: Methionine Deficient Green Florescent Protein (mGFP) is a mutated form of GFPuv (from Aequorea victoria) a GFP variant optimised for maximal fluorescence when excited by UV light. GFPuv can be used as a fusion partner to assist in the expression and isolation of peptides and to monitor biological processes. When GFPuv is fused to other proteins/peptides cyanogen bromide (CNBr) has been used to cleave the fused proteins at methionine residues. However, purification of the protein becomes more complex as the number of methionine residues in the reporter protein increases. GFPuv has four methionine residues which will lead to five fragments in the digestion mixture making purification more difficult. To reduce the downstream burden this mGFP mutant is resistant to CNBr cleavage making the purification process of proteins more efficient.
Additional notes: Methionine Deficient Green Florescent Protein (mGFP) is a mutated form of GFPuv (from Aequorea victoria) a GFP variant optimised for maximal fluorescence when excited by UV light. GFPuv can be used as a fusion partner to assist in the expression and isolation of peptides and to monitor biological processes. When GFPuv is fused to other proteins/peptides cyanogen bromide (CNBr) has been used to cleave the fused proteins at methionine residues. However, purification of the protein becomes more complex as the number of methionine residues in the reporter protein increases. GFPuv has four methionine residues which will lead to five fragments in the digestion mixture making purification more difficult. To reduce the downstream burden this mGFP mutant is resistant to CNBr cleavage making the purification process of proteins more efficient.
Cat. #: 156390
Research Fields: Other
Target: mGFP
References:

Cloning, Fed-Batch Expression And Purification Of A Novel Anti-Candida Peptide And Development Of A Cleavage Resistant Variant Of Green Fluorescence Protein

Related Tools for Vectors

Cat. # Tool Name
152046 pJEK1 Alpha-Synuclein Vector Key Info View Tool
152050 pJEK21 Gamma-Synuclein Vector Key Info View Tool
152593 Norrin Mutant [L61N/A63S] Vector Key Info View Tool
153287 pCMV-iNOS Vector Key Info View Tool
153543 pHyg-AID(1-114) Plasmid Key Info View Tool

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.