RBMXL2 conditional KO mouse

Contributors

Inventor Institute
David Elliott Newcastle University
SKU: 154265
Product description: DNA within a gene is transcribed to make molecules of ribonucleic acid (RNA). The cell then modifies many of these RNAs in a process called splicing before using them as templates to make proteins. The RBMXL2 protein is involved in pre-mRNA processing, metabolism and transport and is only made during and after meiosis in humans and most other mammals. RBMXL2 is primarily localized to the nuclei of meiotic spermatocytes and as such is a candidate gene for autosomal male infertility
Alternate name: RBMX Like 2, Testis-Specific Heterogeneous Nuclear Ribonucleoprotein G-T, RNA-Binding Motif Protein, X-Linked-Like-2, HNRNPGT
Conditional: Yes
Conditional description: Crossing with a Cre expressing mouse is required for knockdown
Production details: A targeting construct in which the Rbmxl2 open reading frame was flanked by LoxP sites was made using standard molecular biology techniques, and electroporated into ES129 cells. Positive clones were injected into blastocysts to create chimaeras and bred to yield agouti pups heterozygous for the targeted locus. The original mice containing the Neomycin gene were crossed to FlpE mice to remove the Neo gene and to generate the Rbmxl2 LoxP conditional allele. Crossing with a Cre expressing mouse is required for knockdown
Cat. #: 154265
Research Fields: Cell biology
Target: RBMXL2
Disease: Male infertility
Model: Conditional KO
Zygosity: Homozygous
Strain: C57BL/6
Phenotype: Male KO mice had smaller testis devoid of sperm. No effect on female fertility
References:

Ehrmann et al. 2019. Elife. 8:. PMID: 30674417

Related Tools for Mouse models

Cat. # Tool Name
151549 Inva3b1 Mouse Key Info View Tool
151454 Bmx Cre ERT2 Mouse Key Info View Tool
151519 BRaf1 KI Mouse Key Info View Tool
151551 Inva2b1 Mouse Key Info View Tool
151554 SAP1 Mouse Key Info View Tool

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.