Raf KI D486A Mouse

Knockin of oncogenic Raf-1D486A; in vivo study of oncogenic Raf-1 mutant, and Ras signalling

Contributors

Inventor Institute
Catrin Pritchard University of Leicester
Cat. #: 151475
Research Fields: Cancer;Cell signaling and signal transduction;Genetics;Neurobiology
Target: Raf1 D486A (oncogenic mutant)
Disease: Cancer
Product description: Knockin of oncogenic Raf-1D486A; in vivo study of oncogenic Raf-1 mutant, and Ras signalling
Conditional: No
Genetic background: A Raf-1 targeting vector, encoding Raf-1 with a substitution at residue 486 (D to A) and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Raf-1 was targeted by homologous recombination with the targeting vector. Correctly targeted ES cells were transfected with a Cre expressing vector to excise the resistance cassette, before injection into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.
Production details: A Raf-1 targeting vector, encoding Raf-1 with a substitution at residue 486 (D to A) and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Raf-1 was targeted by homologous recombination with the targeting vector. Correctly targeted ES cells were transfected with a Cre expressing vector to excise the resistance cassette, before injection into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Kamata et al. 2010. Cancer Res. 70(21):8475-86. PMID: 20978199.

BRAF inactivation drives aneuploidy by deregulating CRAF.

Noble et al. 2008. Mol Cell. 31(6):862-72. PMID: 18922468.

CRAF autophosphorylation of serine 621 is required to prevent its proteasome-mediated degradation.

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