R26LSL-NRL conditional triple-reporter mouse

R26LSL-NRL conditional triple-reporter mouse enabling Cre-activated PET, bioluminescence and fluorescence imaging for tumour tracking and multiscale cell imaging in vivo.

Contributors

Inventor Institute
David Yestin Lewis The Beatson Institute of Cancer Research
SKU: 162485
Product description: R26LSL-NRL conditional triple-reporter mouse enabling Cre-activated PET, bioluminescence and fluorescence imaging for tumour tracking and multiscale cell imaging in vivo.
Alternate name: Rosa26LSL-NRL, R26LSL-NRL, R26NRL, Rosa26 LSL-mNIS-RFP-P2A-Luc2 mouse
Conditional: Yes
Conditional description: Cre-lox conditional reporter system. Cre recombination excises the lox-STOP-lox cassette and activates reporter expression; validated with constitutive CMV-Cre, tamoxifen-inducible R26Cre-ERT2, and tissue-restricted Cre-mediated tumour induction in liver and lung models.
CRISPR: No
Genetic background: The model was generated by targeting HM1 mouse embryonic stem cells with a Rosa26 construct containing CAG-LSL-mNIS-RFP-P2A-Luc2, followed by blastocyst injection into C57BL/6J mice. Experimental animals were maintained on a mixed 129/C57BL/6J background.
Production details: HM1 mouse embryonic stem cells were electroporated with a SwaI-linearised Rosa26 targeting vector containing the CAGSA promoter, lox-STOP-lox NeoR cassette and mNIS-RFP-P2A-Luc2 cDNA. Correctly targeted clones were identified by PCR across both homology arms, then injected into C57BL/6J blastocysts. Germline offspring were identified by coat colour and genotyped using transgene-specific primers.
Cat. #: 162485
Model: Knock-In
Zygosity: Heterozygous
Strain: Mixed 129/C57BL/6J
Phenotype: Viable and inherited in a Mendelian fashion. No overt baseline deleterious phenotype is described; following Cre activation, the allele gives broad functional expression of mNIS, TagRFP and Luc2 across multiple tissues.
Primary citation: Raffo-Iraolagoitia et al. 2026. Nat Biotechnol. Jul 15. doi: 10.1038/s41587-026-03184-3. Epub ahead of print. PMID: 42458052.
Shipping conditions: Sperm / dry ice
References:

Raffo-Iraolagoitia et al. 2026. Nat Biotechnol. Jul 15. doi: 10.1038/s41587-026-03184-3. Epub ahead of print. PMID: 42458052.

Images

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R26LSL-NRL conditional triple-reporter mouse. Schematic of the transgene CAG-LSL-mNIS-RFP-P2A-Luc2 in the Rosa26 locus. Cre recombination deletes the lox-STOP-lox (LSL) sequence and switches on functional mNIS, TagRFP and Luc2 activities in the reporter cell of interest, which mediates corresponding [18F]tetrafluoroborate ([18F]TFB) PET, RFP fluorescence, and luciferin bioluminescence for multimodal imaging.

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