PNPLA1-flox mouse

Useful model for the generation of tissue/cell-specific PNPLA1-deficient mice. Animals might be useful for studies, e.g. on skin development/barrier function or omega-O-acylceramide metabolism, or as model for Autosomal Recessive Congenital Ichthyosis (ARCI).

Contributors

Inventor Institute
Franz Radner University of Graz
Cat. #: 160872
Research Fields: Cell signaling and signal transduction;Metabolism
Target: Patatin-like phospholipase domain containing 1, ichthyosis, ARCI
Model: Conditional KO
Zygosity: Homozygous
Strain: C57BL/6J
Phenotype: These mice are conditional ready, and phenotypical identical to C57BL/6J mice.
Alternate name: PNPLA1
Product description: Useful model for the generation of tissue/cell-specific PNPLA1-deficient mice. Animals might be useful for studies, e.g. on skin development/barrier function or omega-O-acylceramide metabolism, or as model for Autosomal Recessive Congenital Ichthyosis (ARCI).
Conditional: Yes
Conditional description: Targeted conditional ready
Genetic background: These mice were generated using HM1-ES cells. ES cell clones were injected into C57BL/6J blastocysts to enable coat colour selection of the chimeras. Mice were subsequently backcrossed to C57BL/6J (≥ 10 generations). When kept as a homozygous colony, mice are regularly backcrossed to C57BL/6J to avoid generation of subpopulations.
Production details: A floxed neomycin resistance cassette was inserted upstream of exon 1. An additional loxP site was inserted downstream of exon 1. Cre-mediated recombination removed the selection cassette and left Pnpla1 exon 1 floxed. Further details are available upon request.
References:

Grond et al. 2017. J Invest Dermatol. 137(2):394-402. PMID: 27751867.

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