Mimics the expression of D594ABRAF in human cancer samples
| Inventor | Institute |
|---|---|
| Catrin Pritchard | University of Leicester |
| SKU: | 151566 |
|---|---|
| Product description: | Mimics the expression of D594ABRAF in human cancer samples. In conjunction with oncogenic G12DKRAS, this mutant induces the development of highly aggressive malignant melanomas. Conditional knockin (Cre-lox) D594A mutation of BRaf |
| Conditional: | Yes |
| Conditional description: | Cre-lox |
| Genetic background: | A transgenic vector was generated and used to modify the endogenous gene in E14.1a embryonic stem cells by homologous recombination. Targeted ES cells were microinjected into C57BL6 blastocysts and chimaeras were derived. These were bred to C57BL6 mice to obtain germline transmission, from which a colony of heterozygous LSL-BrafD594A mice on the C57BL6 background were derived. |
| Production details: | A transgenic vector was generated and used to modify the endogenous gene in E14.1a embryonic stem cells by homologous recombination. Targeted ES cells were microinjected into C57BL6 blastocysts and chimaeras were derived. These were bred to C57BL6 mice to obtain germline transmission, from which a colony of heterozygous LSL-BrafD594A mice on the C57BL6 background were derived. |
| Cat. #: | 151566 |
|---|---|
| Cancer types: | Skin cancer |
| Research Fields: | Cancer;Genetics |
| Target: | Mouse BRAF |
| Disease: | Cancer |
| Phenotype: | Progressive aneuploidy |
| Shipping conditions: | Embryo/Spermatoza- Dry Ice |
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| References: |
Heidorn et al. 2010. Cell. 140(2):209-21. PMID: 20141835. Kinase-dead BRAF and oncogenic RAS cooperate to drive tumor progression through CRAF. |
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