LFA1 -/- Mouse

In vivo study of LFA-1 knockout; in vivo study of inflammation and leukocyte recruitment;

Contributors

Inventor Institute
Nancy Hogg Cancer Research UK, London Research Institute: Lincoln's Inn Fields
SKU: 151552
Product description: In vivo study of LFA-1 knockout; in vivo study of inflammation and leukocyte recruitment;
Alternate name: LFA-1, Integrin beta-2, Complement receptor C3 subunit beta, CD18
Conditional: No
Genetic background: An LFA-1 targeting vector, containing a resistance cassette inserted into exon 2, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned and injected into C57BL6 blastocysts. Chimeric offspring were backcrossed to establish heterozygotes. LFA+/- mice were interbred to establish LFA-/- homozygotes.
Production details: An LFA-1 targeting vector, containing a resistance cassette inserted into exon 2, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned and injected into C57BL6 blastocysts. Chimeric offspring were backcrossed to establish heterozygotes. LFA+/- mice were interbred to establish LFA-/- homozygotes.
Cat. #: 151552
Research Fields: Genetics; Immunology
Target: Lymphocyte Function-associated Antigen-1 (LFA1)
Disease: Inflammatory disease
Model: Knock-Out
Strain: C57BL/6
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Henderson et al. 2001. J Exp Med. 194(2):219-26. PMID: 11457896.

The use of lymphocyte function-associated antigen (LFA)-1-deficient mice to determine the role of LFA-1, Mac-1, and alpha4 integrin in the inflammatory response of neutrophils.

Berlin-Rufenach et al. 1999. J Exp Med. 189(9):1467-78. PMID: 10224287.

Lymphocyte migration in lymphocyte function-associated antigen (LFA)-1-deficient mice.

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