Chemically-induced skin tumourigenesis model; in vivo study of alpha6 and beta4 integrin transgene expression in skin.
| Inventor | Institute |
|---|---|
| Fiona Watt | Cancer Research UK, London Research Institute: Lincoln's Inn Fields |
| SKU: | 151550 |
|---|---|
| Product description: | Chemically-induced skin tumourigenesis model; in vivo study of alpha6 and beta4 integrin transgene expression in skin. Ninety six percent of alpha6beta4 transgenic mice develop squamous cell carcinoma, compared to 44% wildtype. Of those mice developing tumours, 68% alpha6beta4 develop metastasis, compared to 8% wildtype. TGF-beta signalling is disrupted in alpha6beta4 transgenic mice. |
| Conditional: | Yes |
| Conditional description: | Conditional expression of integrins under involucrin promoter giving tissue-specific suprabasal epidermal expression. |
| Genetic background: | Involucrin – integrin transgene expression constructs were injected into fertilized oocytes from F1 hybrid CBAxC57Bl/6 mice. Transgene-positive mice were back-crossed to generate individual founder lines. alpha6 founder line was crossed with beta4 founder line to generate alpha6beta4 line. |
| Production details: | Involucrin – integrin transgene expression constructs were injected into fertilized oocytes from F1 hybrid CBAxC57Bl/6 mice. Transgene-positive mice were back-crossed to generate individual founder lines. alpha6 founder line was crossed with beta4 founder line to generate alpha6beta4 line. |
| Cat. #: | 151550 |
|---|---|
| Cancer types: | Skin cancer |
| Research Fields: | Cancer; Cell biology; Cell signaling and signal transduction; Genetics; Stem cell biology |
| Target: | Alpha6Beta4 Integrin |
| Disease: | Cancer |
| Zygosity: | Heterozygous |
| Phenotype: | Mice present phenotype very similar to human psoriasis disease. |
| Shipping conditions: | Embryo/Spermatoza- Dry Ice |
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| References: |
Owens DM et al, Journal of Cell Science, 2003, v116 pp3783-3791PMID: 12902406 |
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