This mouse model mimics a chromosomal translocation found in approximately 50% of human DLBCL cases.
| Inventor | Institute |
|---|---|
| Riccardo Dalla-Favera | The Trustees of Columbia University in the City of New York |
| SKU: | 154095 |
|---|---|
| Product description: | Diffuse Large B-Cell Lymphoma (DLBCL) is a B-Cell non-Hodgkins Lymphoma, the fifth most common cancer in the western world. DLBCL is a poorly understood and aggressive disease with ongoing research for the development of effective therapies. This mouse model mimics a chromosomal translocation found in approximately 50% of human DLBCL cases. |
| Alternate name: | Zinc Finger Protein 51, B Cell CLL/Lymphoma 6, Protein LAZ-3 |
| Conditional: | No |
| Production details: | The IÎźHABCL6 targeting vector was constructed by subcloning a HA-tagged murine BCL6 cassette into the pPNT vector, downstream of the IgH IÎź promoter (1.1Kb PCR fragment) and 5Ⲡto a loxP-flanked stop cassette containing a neomycin-resistance gene (neoR). A âź10 Kb EcoRI fragment including the four CÎź exons was then isolated from pEco1.1CÎź vector and subcloned downstream to the neoR cassette. The targeting vector was electroporated in the ES cell line 129/Sv, and Neo-resistant, homologous recombinant clones were identified. After Cre mediated excision of the neoR cassette in vitro by transient transfection of a Cre-expressing plasmid, homologous recombinant ES cell clones were injected into blastocysts from C57BL/6 mice. Chimeric mice obtained from ES clones transmitted the knockin allele through the germline and were all backcrossed onto a C57BL/6 background (1â8 generations) |
| Additional notes: | Knockin mouse expressing BCL6 constitutively in B cells under control of the immunoglobulin IÂľ promotor |
| Cat. #: | 154095 |
|---|---|
| Research Fields: | Cancer; Immunology |
| Target: | BCL6 |
| Disease: | Diffuse Large B-Cell Lymphoma |
| Model: | Knock-In |
| Zygosity: | Homozygous |
| Strain: | C57BL/6 |
| Phenotype: | Knockin mouse expressing BCL6 constitutively in B cells under control of the immunoglobulin I promotor |
| Shipping conditions: | Embryo/Spermatoza- Dry Ice |
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| References: |
Cattoretti et al. 2005. Cancer Cell. 7(5):445-55. PMID: 15894265. Deregulated BCL6 expression recapitulates the pathogenesis of human diffuse large B cell lymphomas in mice. |
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