CDH5(PAC)CreERT2 Mouse

The Cdh5(PAC)-CreERT2 mouse is an ideal tool in the study of gene function in angiogenesis, atherosclerosis and neovascularisation.

Contributors

Inventor Institute
Ralf H. Adams Cancer Research UK, London Research Institute: Lincoln's Inn Fields
SKU: 151520
Product description: The Cdh5(PAC)-CreERT2 mouse model enables efficient inducible conditional recombinase expression in embryonic and adult endothelial cells (tissue-specific loxP knockout/knockin/transgene). The Cdh5(PAC)-CreERT2 mouse is an ideal tool in the study of gene function in angiogenesis, atherosclerosis and neovascularisation.
Alternate name: STOCK Tg(Cdh5-cre/ERT2)1Rha/H
Conditional: Yes
Conditional description: Conditional Cre-ERT2 expression under Cdh5(PAC) promoter in endothelial cells; inducible Cre activity by treatment with hormone (tamoxifen), inducing translocation of Cre-ERT2 to nucleus.
Production details: A Cdh5(PAC)-CreERT2 transgene vector, containing a genomic Cdh5(PAC) promoter fragment fused to a CreERT2 cDNA, was injected into fertilised embryos (C57BL/6 or FVB/N). Founder lines were back-crossed to establish mice heterozygous for the Cdh5(PAC)-CreERT2 transgene.
Additional notes: The Cdh5(PAC)-CreERT2 mouse exhibits tissue-specific expression of an inducible Cre-ERT2 fusion protein, enabling tamoxifen-induced Cre recombinase activity in vascular endothelial cells. Administration of tamoxifen induces nuclear translocation of the Cre-ERT2 fusion protein, and subsequent Cre recombinase activity, allowing knockout/knockin/transgene studies of loxP-flanked genes in vascular endothelial cells. Non-induced Cdh5(PAC)-CreERT2 mice demonstrate no Cre recombinase activity, while tamoxifen-induced Cdh5(PAC)-CreERT2 mice demonstrate high penetrance in vascular endothelial cells (95%+).
Cat. #: 151520
Research Fields: Cancer; Developmental biology; Genetics; Neurobiology; Tissue-specific biology
Target: Estrogen receptor (ERT2) under the vascular endothelial cadherin (Cdh5(PAC)) promoter.
Disease: Cancer
Model: Conditional KO
Zygosity: Heterozygous
Phenotype: The Cdh5(PAC)-CreERT2 mouse exhibits tissue-specific expression of an inducible Cre-ERT2 fusion protein, enabling tamoxifen-induced Cre recombinase activity in vascular endothelial cells.
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

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Robson et al. 2010. Dev Dyn. 239(9):2435-42. PMID: 20652948.

Integration of a Notch-dependent mesenchymal gene program and Bmp2-driven cell…

Citations And Reviews

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