ZR-75-1 HygroR [ZH3D7] cell line

Hygromycin-B-resistant variant of ZR-75-1 human breast-cancer cells

Contributors

Inventor Institute
Lambert Dorssers Erasmus University Medical Center (Erasmus MC)
SKU: 154620
Product description: Hygromycin-B-resistant variant of ZR-75-1 human breast-cancer cells. These cells were used as acceptor cells for somatic cell fusion experiments
Production details: ZR-75-1 cells were transfected with the expression construct PGK-Hyg Br which confers hygromycin B resistance using cationic liposome. Stable transfectants were selected with 60ug/ml of hygromycin B in RBCS medium supplemented with 1nM estradiol.
Additional notes: These cells were used as acceptor cells for somatic cell fusion experiments
Parental cell line: ZR-75-1
Disease: Cancer
Cat. #: 154620
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cancer
Organism: Human
Tissue: Breast
Model: Cancer Model
Cancer Types In Detail: Estrogen dependent;Mammary ductal carcinoma
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: RPMI 1640 medium supplemented with 10% heat-inactivated bovine calf serum (RBCS) and 1 nM 17?-estradiol
Mycoplasma free: Yes
Biosafety level: 1
References:

Dorssers et al. 1997. Int J Cancer. 72(4):700-5. PMID: 9259413.

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