Breast cancer is widely and effectively treated with endocrine treatment. However, in many cases the tumours will eventually progress into an estrogen-independent and therapy-resistant phenotype.Seven genes including AKT1, AKT2, BCAR1, BCAR2, BCAR3, EGFR2 and GRB7 have been shown to directly underlie estrogen independence in human breast cancer cells. This cell line is part of a panel of 16 cell lines (Cat No 154621-154635, 154642) which have been transfected with these genes, plus the parental (Cat No 154547).This cell line is a powerful tool for studying the molecular and cellular mechanisms of breast tumour progression, therapy resistance and to test the effectiveness of novel drugs to combat different modes of anti-estrogen insensitivity
Alternate name:
AKT Serine/Threonine Kinase 2; PKB Beta; Rac Protein Kinase Beta
Production details:
Expression constructs with AKT2 were transfected into ZR-75-1 cells using FuGENE 6 and selected with G418. After selection for G418 resistance colonies were pooled and expanded
Cellosaurus ID:
CVCL_E4WS
Parental cell line:
ZR-75-1
Disease:
Cancer
Cat. #:
154624
Tool sub type:
Continuous
Unit size:
1×10^6 cells / vial
Cancer types:
Breast cancer
Research Fields:
Cancer;Drug development
Organism:
Human
Tissue:
Breast
Model:
Cancer Model
Cancer Types In Detail:
Mammary ductal carcinoma
Format:
Frozen
Storage conditions:
Liquid Nitrogen
Shipping conditions:
Dry ice
Growth medium:
RPMI 1640 medium supplemented with 10% heat-inactivated bovine serum (RBCS) and 1 nM 17?-estradiol
Mycoplasma free:
Yes
Biosafety level:
1
References:
van Agthoven et al. 2009. Breast Cancer Res Treat. 114(1):23-30. PMID: 18351453.
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