ZH3D7:BCAR1 [D4E6] Fusion cell line

This cell line is resistant to hygromycin and Genticin and are maintained in RBCS medium with estradiol and Geneticin. Since they carry a BCAR gene, that can also proliferate slowly in medium without estradiol and supplemented with anti-estrogen.

Contributors

Inventor Institute
Lambert Dorssers Erasmus University Medical Center (Erasmus MC)
SKU: 154640
Product description: Breast cancer is widely and effectively treated with endocrine treatment. However, in many cases the tumours will eventually progress into an estrogen-independent and therapy-resistant phenotype. Somatic cell fusion was used to generate this cell line in order to elucidate the molecular mechanisms underlaying endocrine therapy failure. Using this method revealed that tamoxifen resistance co-segregated with only 1 or 2 of the integration loci present in the tamoxifen-resistant donor cell line, designated the first breast cancer anti-estrogen resistance locus (BCAR1)This cell line is part of a panel of 3 somatic hybrids (Cat No 154639-154641) plus the parental (Cat 154620).This cell line is a powerful tool for studying the molecular and cellular mechanisms of breast tumour progression and therapy resistance.
Alternate name: Breast Cancer Anti-Estrogen Resistance 1; CASS1; P13Cas; CRKAS
Production details: Hygromycin-B-resistant variants of ZR-75-1 cells, ZH3D7, were used as recipients in the somatic-cell-fusion experiments. Donor cells were the anti-estrogen-resistant cell line XI-13. Approximately 6 million donor cells, which were gamma-irradiated with approx 40 Gy and 3 million recipient cells were plated in 25cm flasks in RBCS medium with estradiol. After strong adherence to the flasks in 36 to 48 hr, cells were washed 3 times in RPMI-1640 without serum and incubated with 1 ml polyethylene …
Parental cell line: ZH3D7
Disease: Cancer
Cat. #: 154640
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Breast
Model: Cancer Model
Cancer Types In Detail: Mammary ductal carcinoma
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: R/BCS medium containing estradiol and Geneticin (G418)
Mycoplasma free: Yes
Biosafety level: 1
References:

Brinkman et al. 2000. J Natl Cancer Inst. 92(2):112-20. PMID: 10639512.

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