ZH3D7:BCAR1 [D4E6] Fusion cell line

This cell line is resistant to hygromycin and Genticin and are maintained in RBCS medium with estradiol and Geneticin. Since they carry a BCAR gene, that can also proliferate slowly in medium without estradiol and supplemented with anti-estrogen.

Contributors

Inventor Institute
Lambert Dorssers Erasmus University Medical Center (Erasmus MC)
SKU: 154640
Product description: Breast cancer is widely and effectively treated with endocrine treatment. However, in many cases the tumours will eventually progress into an estrogen-independent and therapy-resistant phenotype. Somatic cell fusion was used to generate this cell line in order to elucidate the molecular mechanisms underlaying endocrine therapy failure. Using this method revealed that tamoxifen resistance co-segregated with only 1 or 2 of the integration loci present in the tamoxifen-resistant donor cell line, designated the first breast cancer anti-estrogen resistance locus (BCAR1)This cell line is part of a panel of 3 somatic hybrids (Cat No 154639-154641) plus the parental (Cat 154620).This cell line is a powerful tool for studying the molecular and cellular mechanisms of breast tumour progression and therapy resistance.
Alternate name: Breast Cancer Anti-Estrogen Resistance 1; CASS1; P13Cas; CRKAS
Production details: Hygromycin-B-resistant variants of ZR-75-1 cells, ZH3D7, were used as recipients in the somatic-cell-fusion experiments. Donor cells were the anti-estrogen-resistant cell line XI-13. Approximately 6 million donor cells, which were gamma-irradiated with approx 40 Gy and 3 million recipient cells were plated in 25cm flasks in RBCS medium with estradiol. After strong adherence to the flasks in 36 to 48 hr, cells were washed 3 times in RPMI-1640 without serum and incubated with 1 ml polyethylene …
Parental cell line: ZH3D7
Disease: Cancer
Cat. #: 154640
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Breast
Model: Cancer Model
Cancer Types In Detail: Mammary ductal carcinoma
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: R/BCS medium containing estradiol and Geneticin (G418)
Mycoplasma free: Yes
Biosafety level: 1
References:

Brinkman et al. 2000. J Natl Cancer Inst. 92(2):112-20. PMID: 10639512.

Images

View Gallery

Related Tools for Cell lines

Cat. # Tool Name
151450 JIM3 Cell Line Key Info View Tool
151626 A375P beta6-puro Cell Line Key Info View Tool
151479 HCT 116 p300 KO [D10] Cell Line Key Info View Tool
151480 HEK293A EGFP-LC3 Cell Line Key Info View Tool
151503 1-7 CE1 Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.