Human follicular dendritic cell immortalised with telomerase, dominant negative TP53 and CDK4(R24C). Transduced to express CD40L and IL-21. Feeder cells for human B cells.
| Inventor | Institute |
|---|---|
| Daniel J Hodson, Rebecca Caeser | University of Cambridge |
| Product description: | Human follicular dendritic cell immortalised with telomerase, dominant negative TP53 and CDK4(R24C). Transduced to express CD40L and IL-21. Feeder cells for human B cells. |
|---|---|
| Conditional: | No |
| Production details: | Folicullar dendritic cells (FDC) were isolated from discarded tonsil tissue and then immortalised through retroviral transduction with pBABE_TERT.Hygro, P53DD_Thy1.1, and CDK4_R24C_Thy1.1, and then further with hCD40Lg-Puro and IL21-LyT2. |
| Receptors of note: | CD40lg |
| Parental cell line: | YK6 |
| Model description: | Cells engineered to express human membrane CD40L |
| Disease: | Cancer |
| Products or characteristics of interest: | Secretion of soluble IL-21 |
| Related tools: | YK6, YK6_CD40L |
| Cat. #: | 162445 |
|---|---|
| Organism: | Human |
| Tissue: | Tonsil |
| Morphology: | Specific dendritic morphology |
| Growth properties: | Adherent |
| Cancer Types In Detail: | Blood cancer |
| Primary citation: | Caeser 2019 Nat Commun PMID: 31586074 |
| Growth medium: | RPMI 1640 |
|---|---|
| Recommended controls: | YK6, YK6_CD40L cell lines |
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