Xist-TetOP mutant: DeltaB+1/2C cell line
Contributors
| Inventor | Institute |
|---|---|
| Sim?Âo Teixeira da Rocha | Instituto de Medicina Molecular Jo?Âo Lobo Antunes |
| SKU: | 160725 |
|---|---|
| Product description: | Deletion of mouse Xist lncRNA to dissect specific RNA modules in the process of X-chromosome inactivation. |
| Conditional: | No |
| Conditional description: | Inducible through addition of doxycycline (1.5 ÄÂÄ?g/ml) to the medium |
| Production details: | CRISPR/Cas9 genome editing of the Xist-TetOP XY ESC line |
| Additional notes: | Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here. This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide these modified CRISPR-Ca… |
| Parental cell line: | Xist-TetOP XY ESCs (Wutz et al., 2002 PMID: 11780141) |
| Cat. #: | 160725 |
|---|---|
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Research Fields: | Genetics; Stem cell biology |
| Organism: | Mouse |
| Model: | Stem Cells |
| Growth properties: | Disruption of Polycomb recruitment to the inactive X-chromosome. |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | ESCs grow in feeder-free classic ESC medium DMEM media containing 15% fetal bovine serum (FBS), 103 U/ml leukemia inhibitor factor (LIF), 10-4 mM 2-mercaptoethanol, 50 U/ml penicillin, and 50 ?g/ml of streptomycin (Gibco) at 37?°C in 5-8% CO2. Medium is changed daily Inducible expression of Xist driven by a TetO promoter is achieved by adding DOX (1.5 ?g/ml). |
| Recommended controls: | Xist-TetOP XY ESCs (Wutz et al., 2002 PMID: 11780141) |
| References: |
Bousard et al. 2019. EMBO Rep. 20(10):e48019. PMID: 31456285. |
|---|
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