Astrocytoma cell line U-251 stably expressing EGFP-LC3 and Mito DsRed for use as a mitophagy sensor.
| Inventor | Institute |
|---|---|
| T.R. Santhosh Kumar | Rajiv Gandhi Centre for Biotechnology |
| SKU: | 161162 |
|---|---|
| Product description: | Astrocytoma cell line U-251 stably expressing EGFP-LC3 and Mito DsRed for use as a mitophagy sensor. |
| Gender: | Male |
| CRISPR: | No |
| Conditional: | No |
| Parental cell line: | U-251MG |
| Disease: | Cancer |
| Cat. #: | 161162 |
|---|---|
| Cancer types: | Brain cancer |
| Research Fields: | Cancer; Disease (not cancer); Metabolism |
| Organism: | Human |
| Tissue: | Brain |
| Gender: | Male |
| Model: | Transgenic |
| Morphology: | Epithelial |
| Growth properties: | Adherent |
| Primary citation: | Lupitha SS, et.al. PMID: 32109534. |
| Initial handling information: | Resuscitation: Rapidly thaw the frozen ampoule in a water bath at 37?°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100-150g for 2-3 minutes to pellet cells (a centrifugation step to remove the cryoprotectant is essential. Seed at the recommend density, e.g. greater than 10,000 cells per cm2. |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry Ice |
| Growth medium: | DMEM with 2mM L-Glutamine and 10% FBS (Heat inactivated). |
| Subculture routine: | Subculture non-confluent cultures using trypsin/EDTA and 1:3 to 1:4, i.e. seeding at 1-4 x10,000 cells/cm? . |
| Temperature: | 37° C |
| Atmosphere: | 5% CO2 |
| Storage medium: | Growth medium + 10% DMSO |
| References: |
Lupitha SS, et.al. PMID: 32109534. |
|---|
Please note we may take up to three days to respond to your enquiry.
CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.