TOV-3392D cell line

Epithelial ovarian cancer cell lines spontaneously derived from solid tumors of patient diagnosed with clear-cell carcinoma suubtype

Contributors

Inventor Institute
Anne-Marie Mes-Masson and Diane Provencher Centre Hospitalier de L’université de Montréal
Cat. #: 161786
Cancer types: Ovarian cancer
Research Fields: Cancer
Organism: Human
Tissue: Derived from solid tumor of patient diagnosed with epithelial ovarian cancer with a clear cell carcinoma subtype
Gender: Female
Morphology: Characteristic flat epithelial morphology, grew in dense clusters, and gained little confluence
Growth properties: Adherent
Primary citation: Sauriol A et al. 2020. Cancers. 12(8):2222. PMID: 32784519
Alternate name: TOV-3392D
Product description: Epithelial ovarian cancer cell lines spontaneously derived from solid tumors of patient diagnosed with clear-cell carcinoma suubtype. Sample was taken from the right ovary. Cell line produces tumors in mice upon SC injection
Gender: Female
CRISPR: No
Production details: Established using the Scrape method where tumor tissue was scraped into a 100mm plate with complete OSE medium and maintained for 40 days with medium replaced weekly.
Additional notes: Patient 3392 had previous personal history of breast cancer and was naive to ovarian cancer treatment but received prior chemotherapy for breast cancer. Patient was age 42 at diagnosis and har prior history of breast cancer. Treatment included 5-fluorouracil, epirubicin, cyclo-phosphamide, radiotherapy, and trastuzumab. Sampling was done during 2007.
Receptors of note: No
Growth medium: OSE medium contains 10% FBS, 0.5ug/mL amphotericin B and 50 ug/mL gentamicin
Temperature: 37° C
Atmosphere: Low oxygen conditions of 7% O2 and 5% CO2
References:

Gaia-Oltean et al. 2021. Exp Ther Med. 21(3):243. PMID: 33603851 Canals Hernaez et al. 2020. J Immunother Cancer. 8(2):e001128. PMID: 33243933 Borzdzi?owska et al. 2022. Biomedicines. 11

10(5):1116. PMID: 35625852 Meng et al. 2023. J Anim Sci. 3

101:skad215. PMID: 37351870 Koni et al. 2020. Int J Mol Sci. 18

21(20):7697. PMID: 33080952

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