| Inventor | Institute |
|---|---|
| Jorgen Fogh | Memorial Sloan-Kettering Cancer Center (MSK) |
| SKU: | 161909 |
|---|---|
| Product description: | Human Carcinoma Cell Line Derived from a Lung Metastatic Site. |
| Gender: | Male |
| Cellosaurus ID: | CVCL_2777 |
| Cat. #: | 161909 |
|---|---|
| Cancer types: | Lung cancer |
| Organism: | Human |
| Tissue: | Lung |
| Gender: | Male |
| Morphology: | Epithelial |
| Growth properties: | Adherent |
| Primary citation: | Fogh et al. 1977. Journal of the National Cancer Institute. 59: 221-226. PMID: 327080. |
| Storage conditions: | Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability. |
|---|---|
| Shipping conditions: | Dry Ice |
| Growth medium: | McCoy’s 5a Medium Modified supplemented with 15% FBS |
| Subculture routine: | Subcultures are prepared by scraping. Remove spent medium, add fresh, dislodge cells from the floor of the flask, aspirate to disperse and dispense into new flasks. Subculture about once a week. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week |
| Temperature: | 37° C |
| Atmosphere: | 95% Air, 5% CO2 |
| Storage medium: | Culture medium 95%; DMSO, 5% |
| Biosafety level: | 1 |
| References: |
Fogh J et al. 1977. Journal of the National Cancer Institute. 59: 221-226. PMID: 327080. |
|---|
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