Human Carcinoma Cell Line Derived from a Lung Metastatic Site.
Gender:
Male
Cellosaurus ID:
CVCL_2777
Cat. #:
161909
Cancer types:
Lung cancer
Organism:
Human
Tissue:
Lung
Gender:
Male
Morphology:
Epithelial
Growth properties:
Adherent
Primary citation:
Fogh et al. 1977. Journal of the National Cancer Institute. 59: 221-226. PMID: 327080.
Storage conditions:
Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Shipping conditions:
Dry Ice
Growth medium:
McCoy’s 5a Medium Modified supplemented with 15% FBS
Subculture routine:
Subcultures are prepared by scraping. Remove spent medium, add fresh, dislodge cells from the floor of the flask, aspirate to disperse and dispense into new flasks. Subculture about once a week. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week
Temperature:
37° C
Atmosphere:
95% Air, 5% CO2
Storage medium:
Culture medium 95%; DMSO, 5%
Biosafety level:
1
References:
Fogh J et al. 1977. Journal of the National Cancer Institute. 59: 221-226. PMID: 327080.
Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.
Please note we may take up to three days to respond to your enquiry.
CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.