Colon cancer cell line stably transfected with EGFP
| Inventor | Institute |
|---|---|
| Stefan Przyborski | University of Durham |
| SKU: | 162203 |
|---|---|
| Product description: | Colon cancer cell line stably transfected with EGFP. Suitable for studying the effect of the absence of lamin A on cancer cell properties. Control for SW480/lamA and SW480-EGP emerin transfected cell lines. |
| Alternate name: | SW480 GFP-alone 1; SW480 GFP1 |
| Receptors of note: | No |
| Parental cell line: | SW480 |
| Cat. #: | 162203 |
|---|---|
| Organism: | Human |
| Tissue: | Colon |
| Model: | Genetically modified cell line |
| Morphology: | Epithelial |
| Growth properties: | Adherent |
| Initial handling information: | Continue to store at ?-70deg C. To bring up cells, defrost quickly in 37?C water bath and seed in petri dish/flask. Once cells have adhered to culture surface, change media to DMSO-free media. |
|---|---|
| Format: | Frozen |
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | L15 (Leibovitz) medium with 2mM L-Glutamine, 10% FBS and 100units/ml penicillin / 100ug/ml streptomycin |
| Subculture routine: | Passage at 70-80% confluencey in the presence of 0.25% trypsin in 0.5mM EDTA/PBS. Incubate at 37?C, 5% Co2, humidified for 2 minutes. Split 1:4. |
| Atmosphere: | Without CO2 |
| Storage medium: | L15 (Leibovitz) medium with 2mM L-Glutamine, 10% FBS and 10% DMSO |
| Recommended controls: | Same cell line stably transfected with EGFP not tagged to any other DNA |
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