SW48-PLCG1

For stable PLCG1 overexpression (SW48-PLCG1), SW48 cells were infected with PLCG1 lentiviral activation particles (Santa Cruz Biotechnology). Selection of stable clones started 2 days after infection with a mix of 2 ?g/mL Puromycin, 50 mg/mL Hygromicyn B, and 10 mg/mL Blasticidin.

Contributors

Inventor Institute
Marta Martins Instituto de Medicina Molecular João Lobo Antunes
Cat. #: 161945
Cancer types: Colorectal cancer
Research Fields: Cancer
Organism: Human
Tissue: Epithelial
Gender: Male
Model: Over-expression
Growth properties: A subcultivation ratio of 1:4 to 1:6 is recommended, 1 to 2 times per week.
Primary citation: Martins, et al. 2022. Clin Cancer Res. 28(6):1203-1216. PMID: 34980600
Product description: For stable PLCG1 overexpression (SW48-PLCG1), SW48 cells were infected with PLCG1 lentiviral activation particles (Santa Cruz Biotechnology). Selection of stable clones started 2 days after infection with a mix of 2 ?g/mL Puromycin, 50 mg/mL Hygromicyn B, and 10 mg/mL Blasticidin.
Gender: Male
CRISPR: No
Parental cell line: SW480
Disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry Ice
Growth medium: DMEM with 10% FBS, 5% CO2
Recommended controls: shRNA scrambled control
Biosafety level: 1
References:

Martins, et al. 2022. Clin Cancer Res. 28(6):1203-1216. PMID: 34980600

Related Tools for Cell lines

Cat. # Tool Name
151463 Omega-E Packaging Cell Line Key Info View Tool
151631 C-Raf KI D486A Cell Line Key Info View Tool
151449 JIM1 Cell Line Key Info View Tool
151450 JIM3 Cell Line Key Info View Tool
151558 MEF Trex1 KO Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.