For stable PLCG1 overexpression (SW48-PLCG1), SW48 cells were infected with PLCG1 lentiviral activation particles (Santa Cruz Biotechnology). Selection of stable clones started 2 days after infection with a mix of 2 ?g/mL Puromycin, 50 mg/mL Hygromicyn B, and 10 mg/mL Blasticidin.
Gender:
Male
CRISPR:
No
Parental cell line:
SW480
Disease:
Cancer
Cat. #:
161945
Cancer types:
Colorectal cancer
Research Fields:
Cancer
Organism:
Human
Tissue:
Epithelial
Gender:
Male
Model:
Over-expression
Growth properties:
A subcultivation ratio of 1:4 to 1:6 is recommended, 1 to 2 times per week.
Primary citation:
Martins, et al. 2022. Clin Cancer Res. 28(6):1203-1216. PMID: 34980600
Format:
Frozen
Storage conditions:
Liquid Nitrogen
Shipping conditions:
Dry Ice
Growth medium:
DMEM with 10% FBS, 5% CO2
Recommended controls:
shRNA scrambled control
Biosafety level:
1
References:
Martins, et al. 2022. Clin Cancer Res. 28(6):1203-1216. PMID: 34980600
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