SK-MEL-5 Cell Line

Contributors

Inventor Institute
Lloyd J. Old, Toshitada Takahashi Memorial Sloan-Kettering Cancer Center (MSK)
SKU: 161920
Product description: SK-MEL-5 is one of a series of melanoma cell lines established from patient-derived tumour samples. This cell line expresses mutant B-Raf (V600E) and wildtype N-Ras.
Gender: Female
Model description: Tumourigenic in nude mice; forms malignant melanoma
Products or characteristics of interest: Karyotype: modal number = 120; range = 95 to 133. This is a hyperpentaploid human cell line with the modal chromosome number of 120, occurring in 9% of cells. The majority of cells had chromosome counts of 114-123. Among 14 marker chromosomes that were common to most cells, generally 9q+ had four copies per cell, t(1q8q) and del(5) (q34) had three copies per cell, and 14q+ had two copies per cell. Numerous others were also found in single copies, all infrequently. Three normal X chromosomes were found in most cells, N9 was absent, and N7 and N20 had seven to eight copies per cell. Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1-2; PGM1, 1-2; PGM3, 1
Cat. #: 161920
Cancer types: Skin cancer
Organism: Human
Tissue: Skin
Gender: Female
Model: Tumourigenic
Morphology: Stellate
Growth properties: Adherent
Primary citation: Carey et al. 1976. Proceedings of the National Academy of Sciences. 73: 3278-3282. PMID: 1067619.
Storage conditions: Vapor phase of liquid nitrogen
Shipping conditions: Dry Ice
Growth medium: EMEM supplemented with FBS to a final concentration of 10%
Temperature: 37° C
Atmosphere: 5% CO2 in air
Storage medium: Complete growth medium, 95%; DMSO, 5%
Biosafety level: 1
References:

Xing et al. 2012. Oncogene. 31(4):446-457. PMID: 21725359.

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