| Inventor | Institute |
|---|---|
| Lloyd J. Old, Toshitada Takahashi | Memorial Sloan-Kettering Cancer Center (MSK) |
| SKU: | 161919 |
|---|---|
| Product description: | SK-MEL-31 is one of a series of melanoma cell lines established from patient-derived tumour samples. This cell line expresses wildtype B-Raf and wildtype N-Ras. |
| Gender: | Female |
| Cellosaurus ID: | CVCL_0600 |
| Model description: | Tumourigenic in nude mice; produces malignant melanoma |
| Products or characteristics of interest: | Karyotype: modal number = 82; range = 74 to 84. This is a hypotetra-/hypertriploid human cell line. The modal chromosome number is 82 occurring at 16% with polyploidy at 10.3%. The markers del(1)(q23), t(1q10p), del(7)(p13), t(7p18q), i(21q) and 5 others are common to all cells. They are all paired in most cells. N1 and N18 are absent, N11 is single-copied, and N6, N7, N8 and N16 are generally 4-copied (many cells also had 5 copies each for N7 and N8). The X is paired. Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1-2; PGM1, 1; PGM3, 1 |
| Cat. #: | 161919 |
|---|---|
| Cancer types: | Skin cancer |
| Organism: | Human |
| Tissue: | Skin |
| Gender: | Female |
| Model: | Tumourigenic |
| Morphology: | Epithelial |
| Growth properties: | Adherent |
| Primary citation: | Carey et al. 1976. Proceedings of the National Academy of Sciences. 73: 3278-3282. PMID: 1067619. |
| Storage conditions: | Vapor phase of liquid nitrogen |
|---|---|
| Shipping conditions: | Dry Ice |
| Growth medium: | EMEM supplemented with 2 mM L-glutamine and Earle’s BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 85%; fetal bovine serum, 15% |
| Temperature: | 37° C |
| Atmosphere: | 5% CO2 in air |
| Storage medium: | Complete growth medium supplemented with 5% (v/v) DMSO |
| Biosafety level: | 1 |
| References: |
Xing et al. 2012. Oncogene. 31(4):446-457. PMID: 21725359. |
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