| Inventor | Institute |
|---|---|
| Lloyd J. Old, Toshitada Takahashi | Memorial Sloan-Kettering Cancer Center (MSK) |
| SKU: | 161916 |
|---|---|
| Product description: | K-MEL-24 is one of a series of melanoma cell lines established from patient-derived tumour samples. This cell line expresses wildtype B-Raf and wildtype N-Ras. |
| Gender: | Male |
| Cellosaurus ID: | CVCL_0599 |
| Model description: | Tumourigenic; forms malignant melanoma |
| Products or characteristics of interest: | Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1-2; Me-2, 2; PGM1, 1; PGM3, 2 |
| Cat. #: | 161916 |
|---|---|
| Cancer types: | Skin cancer |
| Organism: | Human |
| Tissue: | Skin |
| Gender: | Male |
| Model: | Tumourigenic |
| Morphology: | Stellate |
| Growth properties: | Adherent |
| Cancer Types In Detail: | Melanoma |
| Primary citation: | Carey et al. 1976. Proceedings of the National Academy of Sciences. 73: 3278-3282. PMID: 1067619. |
| Storage conditions: | Vapor phase of liquid nitrogen |
|---|---|
| Shipping conditions: | Dry Ice |
| Growth medium: | EMEM supplemented with FBS to a final concentration of 15% |
| Subculture routine: | Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking |
| Temperature: | 37° C |
| Atmosphere: | 5% CO2 in air |
| Storage medium: | Complete growth medium supplemented with 5% (v/v) DMSO |
| Biosafety level: | 1 |
| References: |
Xing et al. 2012. Oncogene. 31(4):446-457. PMID: 21725359. |
|---|
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