| Inventor | Institute |
|---|---|
| Herbert F. Oettgen | Memorial Sloan-Kettering Cancer Center (MSK) |
| SKU: | 161914 |
|---|---|
| Product description: | SK-MEL-1 are melanocytes established from patient-derived tumour samples. This cell line expresses mutant B-Raf (V600E) and wildtype N-Ras and were isolated from the skin of a 29-year-old, White male patient with melanoma. |
| Gender: | Male |
| Products or characteristics of interest: | Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis. Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases. |
| Related tools: | – |
| Cat. #: | 161914 |
|---|---|
| Cancer types: | Skin cancer |
| Tissue: | Skin |
| Gender: | Male |
| Morphology: | Spherical |
| Growth properties: | Clusters in suspension |
| Cancer Types In Detail: | Melanoma |
| Primary citation: | Oettgen et al. 1968. Journal of the National Cancer Institute. 41: 827-843. PMID: 4879578. |
| Storage conditions: | Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability. |
|---|---|
| Shipping conditions: | Dry Ice |
| Growth medium: | EMEM suplemented with 10% FBS. |
| Subculture routine: | Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 1 x 105 cells/mL and maintain at 2 x 105 cells/mL. Medium Renewal: 2 to 3 times per week |
| Temperature: | 37° C |
| Storage medium: | Complete growth medium, 95%; DMSO, 5% |
| Recommended controls: | – |
| Biosafety level: | 1 |
| References: |
Xing et al. 2012. Oncogene. 31(4):446-457. PMID: 21725359. Fogh et al. 1977. Journal of the National Cancer Institute. 59: 221-226. PMID: 327080. Oettgen et al. 1968. Journal of the National Cancer Institute. 41: 827-843. PMID: 4879578. |
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