SK-LMS-01 Cell Line

Contributors

Inventor Institute
Germain Trempe Memorial Sloan-Kettering Cancer Center (MSK)
SKU: 161912
Product description: SK-LMS-1 is a cell line exhibiting fibroblast morphology that was isolated from the vulva of a 43-year-old, White female patient with leiomyosarcoma
Gender: Female
Products or characteristics of interest: The cell line is aneuploid human female, with chromosome counts in the triploid to hypertriploid range. Neither of the X chromosomes are normal, but their q arms are detected in a translocation with chromosome N4. Normal chromosomes N1, N4, N13, and N17 are clearly under-represented, while chromosomes N6, N18, and N20 are consistently over-represented with respect to the copy numbers of other normal chromosomes. Eight marker chromosomes are identified: 5p+, t(1pter—>cen::9q12—>9q33::3p21—>3pter), t(1p;?), der(2)t(1;2)(q21;q24), 12p+, der(6)t(6;?)(q21;?), der(4)t(X;4)(q13;q34), del(16)(p13). The cytogenetic and isozyme phenotype descriptors are in accord with those given by J. Fogh.
Cat. #: 161912
Cancer types: Uterine cancer
Organism: Human
Tissue: Vulva
Gender: Female
Morphology: Fibroblast
Growth properties: Adherent
Primary citation: Fogh J., et al. 1975. Springer; New York.115-159.
Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Shipping conditions: Dry Ice
Growth medium: EMEM suplemented with 10% FBS.
Temperature: 37° C
Storage medium: Complete growth medium, 95%; DMSO, 5%
Biosafety level: 1

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