SK-BR-3 Cell Line

Human breast cancer cell line that overexpresses the Her2 (Neu/ErbB-2) gene product. These cells display an epithelial morphology in tissue culture.

Contributors

Inventor Institute
Lloyd J. Old, Germain Trempe Memorial Sloan-Kettering Cancer Center (MSK)
SKU: 161911
Product description: SK-BR-3 is a human breast cancer cell line that overexpresses the Her2 (Neu/ErbB-2) gene product. These cells display an epithelial morphology in tissue culture and are capable of forming poorly differentiated tumours in immunocompromised mice. The SK-BR-3 cells and products derived from it are used often as positive controls in assays for Her2. In addition, the cell line is also a useful preclinical model to screen for therapeutic agents targeting Her2 and to delineate mechanisms of resistance to Her2-targeted therapies.
Gender: Male
Cat. #: 161911
Tool sub type: Continuous
Cancer types: Breast cancer
Organism: Human
Tissue: Breast; Mammary gland
Gender: Male
Morphology: Epithelial; Ultrastructural features include microvilli and desmosomes, glycogen granules, large lysosomes, bundles of cytoplasmic fibrils.
Growth properties: Adherent
Primary citation: Fogh J et al. 1977. Journal of the National Cancer Institute. 59: 221-226.PMID: 327080.
Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Shipping conditions: Dry Ice
Growth medium: McCoy’s 5a Medium Modified supplemented with 10% FBS
Temperature: 37° C
Atmosphere: 95% Air, 5% CO2
Storage medium: Complete growth medium, 95%; DMSO, 5%
Biosafety level: 1
References:

Fogh J et al. 1977. Journal of the National Cancer Institute. 59: 221-226.PMID: 327080.

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