SIRMu-1 Cell Line
Contributors
| Inventor | Institute |
|---|---|
| Daniel Peet | The University of Adelaide |
| SKU: | 154858 |
|---|---|
| Product description: | SIRMu-1 Cell Line: Both the cellular morphology and overall transcriptome of the SIRMu-1?cells are more similar to primary rat MCs than the commonly used rMC-1?cells |
| Production details: | A monoclonal line dervied from two sequential rounds of single cell cloning by serial dilution. No Virus or any pathogen. Not GMO. The cells have not been transformed with any agents. The cells can be passaged 1:3 to 1:5 every 2-4 days. Estimated population doubling times with cultured in 10% and 20% FBS are 36 and 30 hours respectively. The cells can take time to recover after thawing from a frozen stock. After thawing, it is quite normal to have a lot of dead and floating cells. For a vial… |
| Cellosaurus ID: | CVCL_UW38 |
| Disease: | Retinal studies into Muller cell function |
| Cat. #: | 154858 |
|---|---|
| Unit size: | 1×10^6 cells / vial |
| Organism: | Rat |
| Tissue: | Eye |
| Model: | Immortalised Line |
| Growth properties: | large and flat with a “ghost-like” appearance. |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | MEM*, 20% FBS, 25mM glucose (final concentration), 2mM L-glutamine. *Minimum Essential Medium (+Earle’s Salts, -L glutamine, Gibco #11090). Note: The cells also grow fine in 10% FBS and/or 5mM glucose. |
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