S_N40R2 Cell Line
Exclusive toolContributors
| Inventor | Institute |
|---|---|
| John Lunec | Newcastle University |
| SKU: | 152838 |
|---|---|
| Product description: | To determine how resistance to MDM2/p53 binding antagonists might develop, SJSA-1 cells were exposed to growth inhibitory concentrations of MDM2 inhibitors, Nutlin-3, and a clonal resistant cell line generated. The p53 mediated responses of parental and resistant cell lines were compared. In contrast to the parental cell lines, p53 activation by Nutlin-3, MI-63 or ionizing radiation was not observed in the SJSA-1 derived cell lines. |
| Conditional: | No |
| Production details: | Resistant cell lines were established by exposing SJSA-1 cells to Nutlin-3. Single cell derived colonies were isolated with cloning cylinders and the clonal population expanded in culture medium containing the MDM2/p53 antagonist refreshed weekly for 60 days. Stage 1 resistant clones were then further exposed to increased concentrations of Nutlin-3 for 30 days to generate stage 2 resistant clones. |
| Cellosaurus ID: | CVCL_HG10 |
| Parental cell line: | SJSA-1 |
| Disease: | Cancer |
| Cat. #: | 152838 |
|---|---|
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Sarcoma |
| Research Fields: | Apoptosis and autophagy;Cancer;Cell biology;Drug development;Genetics |
| Tissue: | Bone |
| Model: | Cancer Model |
| Growth properties: | Adherent |
| Cancer Types In Detail: | Multipotential sarcoma;Osteosarcoma |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
TP53 mutant MDM2-amplified cell lines selected for resistance to MDM2-p53 binding antagonists retain sensitivity to ionizing radiation. Drummond et al. 2016. Oncotarget. :. PMID: 27323823. |
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S_N40R2 Cell Line. Early log phase
S_N40R2 Cell Line. Early log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
