S_M6R1 Cell Line

To determine how resistance to MDM2/p53 binding antagonists might develop, SJSA-1 cells were exposed to growth inhibitory concentrations of a MDM2 inhibitor, MI-63, and a clonal resistant cell line was generated.

Contributors

Inventor Institute
John Lunec Newcastle University
SKU: 152839
Product description: To determine how resistance to MDM2/p53 binding antagonists might develop, SJSA-1 cells were exposed to growth inhibitory concentrations of a MDM2 inhibitor, MI-63, and a clonal resistant cell line was generated.
Conditional: No
Production details: Resistant cell lines were established by exposing SJSA-1 cells to MI-63. Single cell derived colonies were isolated with cloning cylinders and the clonal population expanded in culture medium containing the MDM2/p53 antagonist refreshed weekly for 60 days. Stage 1 resistant clones were then further exposed to increased concentrations of MI-63 for 30 days to generate stage 2 resistant clones.
Cellosaurus ID: CVCL_HG09
Parental cell line: SJSA-1
Disease: Cancer
Cat. #: 152839
Provenance and Ownership: The S_M6R1 Cell Line is owned by Cancer Research UK. CancerTools supplies the S_M6R1 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing S_M6R1 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Apoptosis and autophagy; Cancer; Cell biology; Drug development; Genetics
Organism: Human
Tissue: Bone
Model: Cancer Model
Cancer Types In Detail: Multipotential sarcoma;Osteosarcoma
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Mycoplasma free: Yes
Biosafety level: 1
References:

TP53 mutant MDM2-amplified cell lines selected for resistance to MDM2-p53 binding antagonists retain sensitivity to ionizing radiation.

Drummond et al. 2016. Oncotarget. :. PMID: 27323823.

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