RT112/84 Cell Line
Exclusive toolA patient-derived bladder cancer cell line used as a model of low-invasive disease to study urothelial differentiation, tumour invasion, and bladder cancer progression. Only available through CancerTools. Save 30% on this cell line this autumn. Use code ACA26CELL at checkout.
Contributors
| Inventor | Institute |
|---|---|
| Chris Marshall | Cancer Research UK, London Research Institute: Lincoln's Inn Fields |
| SKU: | 153250 |
|---|---|
| Product description: | The RT112/84 cell line is derived from human bladder carcinoma. This cell line is a slow growing tumour which does not form colonies in soft agar. RT1112/84 cells requires 400 cells/dish to form colonies. RT112/84 is tumourigenic in nude mice. This cell line expresses high levels of E-cadherin, alpha-, beta-, and gamma-catenins. |
| Alternate name: | RT112-84; RT-112/84; RT11284 |
| Gender: | Female |
| Additional notes: | STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 13,14 D16S539: 11,13 D5S818: 10,13 D7S820: 11,12 THO1: 7 TPOX: 8,11 vWA: 14,17 |
| Cellosaurus ID: | CVCL_2714 |
| Disease: | Cancer |
| Cat. #: | 153250 |
|---|---|
| Provenance and Ownership: | The RT112/84 Cell Line is owned by Cancer Research UK. CancerTools supplies the RT112/84 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing RT112/84 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Bladder cancer |
| Organism: | Human |
| Tissue: | Bladder |
| Gender: | Female |
| Model: | Tumour line |
| Growth properties: | Adherent |
| Cancer Types In Detail: | Human bladder carcinoma epithelial |
| Primary citation: | Marshall et al. 1977. J Natl Cancer Inst. 58(6):1743-51. PMID: 864752. |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS). |
| Subculture routine: | Split sub-confluent cultures (70-80%) seeding at 2-4×10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Subculture weekly – cells are difficult to trypsinise, and should be kept sub-confluent. |
| References: |
Marshall et al. 1977. J Natl Cancer Inst. 58(6):1743-51. PMID: 864752. |
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RT112/84 Cell Line.
RT112/84 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)
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