RCC-FG2 + PBRM1 Clone 13

RCC-FG2 cell line deficient for PBRM1 stably expressing DOX-inducible PBRM1 construct (Clone 13)

Contributors

Inventor Institute
Jessica Downs, Hugang Feng The Institute of Cancer Research
SKU: 161867
Product description: RCC-FG2 cell line deficient for PBRM1 stably expressing DOX-inducible PBRM1 construct (Clone 13). This cell line was generated using CRISPR-Cas9 engineering to create a loss of function mutation in the indicated subunit of the SWI/SNF chromatin remodelling complex. The cell line was validated using Sanger sequencing, immunofluorescence, and proteomic analysis.
Gender: Male
CRISPR: Yes
Conditional: Yes
Conditional description: Conditional expression of wild type PBRM1 gene following deoxycycline treatment
Cellosaurus ID: CVCL_5873
Parental cell line: RCC-FG2
Model description: Conditional re-expression of wild type PBRM1 gene
Products or characteristics of interest: Gene loss-of-function, chromatin-remodelling, DNA damage, cell cycle response, regulation of t-cell-mediated cell killing.
Cat. #: 161867
Cancer types: Renal cancer
Research Fields: Cancer
Organism: Human
Tissue: kidney
Gender: Male
Model: Genetically modified
Morphology: Epithelial
Growth properties: Adherent
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: RPMI-1640 + 10S + 1% Penicillin/Streptomycin
Temperature: 37C
Atmosphere: 5% CO2 in air
Biosafety level: 1
References:

Unpublished

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