PC3#33 rho-0 cell line

PC3 cell line lacking mitochondrial DNA and oxidative phosphorylation

Contributors

Inventor Institute
Mikhail Alexeyev University of South Alabama Foundation for Research and Commercialization
Cat. #: 162187
Cancer types: Prostate cancer
Organism: Human
Gender: Male
Model: Genetically modified cell line
Morphology: Epithelial
Growth properties: Adherent
Primary citation: Khozhukhar et al. 2018. Curr Protoc Cell Biol. PMID: 30040188
Product description: PC3 cell line lacking mitochondrial DNA and oxidative phosphorylation. Auxotrophic for uridine and pyruvate. Can be used for generation of transmitochondrial cybrids, control in experiments studying mtDNA contribution to tumor initiation, promotion, malignant conversion, and progression.
Gender: Male
CRISPR: No
Conditional: No
Production details: Prolonged incubation with an inhibitor of mtDNA replication followed by cloning
Additional notes: Cells rely exclusively on glycolysis for their growth. Therefore, they tend to consume glucose faster than their WT counterparts, acidify the medium faster, and die as soon as they consume all glucose. Therefore, do not allow excessive medium acidification (an indicator of glucose depletion) and do not grow cells to high densities
Parental cell line: PC3
Model description: Lacks mitochondrial DNA and all genes encoded there
Disease: Mitochondrial Disease
Initial handling information: Upon arrival, store the vial in liquid nitrogen. Quickly thaw the vial in a 37C water bath, transfer vial's content into a 15-ml centrifuge tube containing complete growth medium, pellet cells by centrifugation, remove the medium, resuspend the cells in 10 ml of prewarmed complete growth medium, transfer to 100-mm TC-treated petri dish, incubate @37C+5% CO2.
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM medium with 4.5 g/l glucose supplemented with 10% FBS, 50 ug/ml gentamycin, 50 ug/ml uridine and 1 mM sodium pyruvate
Subculture routine: Do not grow to confluence and do not allow medium acidification. When the growth medium starts changing its color, immediately change the medium or passage cells. To passage, aspirate growth medium, wash with PBS (without Ca++ or Mg++), aspirate PBS, add 0.05% Trypsin in PBS, incubate @37C, 5% CO2 for 5-10 min, tap the vessel to dislodge cells, neutralize trypsin by adding 5 volumes of complete growth medium, disperse cells by trituration, plate 1:4 to 1:10 dilution
Atmosphere: 5% CO2
Storage medium: (DMEM medium with 4.5 g/l glucose supplemented with 10% FBS, 50 ug/ml gentamicin, 50 ug/ml uridine and 1 mM sodium pyruvate)=45% + (Heat-inactivated newborn calf serum)=45% + 10% sterile DMSO
Recommended controls: WT PC3 cells
References:

Khozhukhar et al. 2018. Curr Protoc Cell Biol. PMID: 30040188

Related Tools for Cell lines

Cat. # Tool Name
151463 Omega-E Packaging Cell Line Key Info View Tool
151559 MEF UNG KO Cell Line Key Info View Tool
151629 MEF Raf1 FF/FF KI Cell Line Key Info View Tool
151663 MEF PKCe KO KI Cell Line Key Info View Tool
151014 MCF7 AREc32 Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.