NHE-1 expressing PS120 cell line

An isoform of the Na+/H+ exchanger, NHE-1, has been cloned, sequenced and stably expressed in PS120 fibroblast cells. PS120 being a Na+/H+ exchanger deficient derivative of the CCL39 cell line, poses no background in experimental studies. The NHE cDNA was cloned and sequenced from a rabbit ileal villus cell cDNA library. The cells were then stably transfected using double selection with G418 and acid selection. The Na+/H+ exchange activity of these cells were proven using fluorescence measurement using a pH sensitive dye. These cells are ideal for the Na+/H+ exchanger functional studies as well as drug effect studies with null…

Contributors

Inventor Institute
Mark Donowitz Johns Hopkins University
SKU: 156412
Product description: An isoform of the Na+/H+ exchanger, NHE-1, has been cloned, sequenced and stably expressed in PS120 fibroblast cells. PS120 being a Na+/H+ exchanger deficient derivative of the CCL39 cell line, poses no background in experimental studies. The NHE cDNA was cloned and sequenced from a rabbit ileal villus cell cDNA library. The cells were then stably transfected using double selection with G418 and acid selection. The Na+/H+ exchange activity of these cells were proven using fluorescence measurement using a pH sensitive dye. These cells are ideal for the Na+/H+ exchanger functional studies as well as drug effect studies with null background. Additional advantages include:â?‚€?‹Â˜ NHE-1 aids in the development of treatment for cardiac ischemia
Parental cell line: CCL39
Disease: Cardiac ischemia
Cat. #: 156412
Unit size: 1×10^6 cells / vial
Cancer types: Lung cancer
Research Fields: Cell signaling and signal transduction;Drug development
Format: Frozen
Shipping conditions: Dry ice

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